Screening of paralytic shellfish poisoning toxins in naturally occurring samples with three different direct competitive enzyme-linked immunosorbent assays
Screening of paralytic shellfish poisoning toxins in naturally occurring samples with three different direct competitive enzyme-linked immunosorbent assays
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DOI：
10.1021/jf960244w
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发表时间：
1996-12-01
影响因子：
6.1
通讯作者：
Allison, C
中科院分区：
文献类型：
--
作者：
Chu, FS;Hsu, KH;Allison, C
在3种直接竞争酶联免疫吸附试验（dc-ELISA）中，以抗石房蛤毒素（STX）/STX-HRP或抗新石房蛤毒素/STX-HRP对检测麻痹性贝毒（PSP）最为有效。在80 μ g/100 g样品的阈值水平下，对1540份自然污染的样品进行分析，发现ELISA法检测的PSP总毒素（STX+新STX水平）与小鼠法检测的PSP总毒素（STX+新STX水平）之间有很好的一致性，ELISA法检测的211份样品（13.6%）为阳性，而小鼠法检测的175份样品（11.3%）为阳性。提供了通过不同ELISA形式获得的1540份样品中>40和>80 μ g/100 g水平的毒素分布。通过ELISA同时分析STX和neo-STX获得的总PSP毒素水平（STX加neo-STX）对于准确筛选这些样品中PSP毒素的存在是有效的，并且可以消除运行小鼠测定所需的多达80-85%的样品。
Among three direct competitive enzyme-linked immunosorbent assays (dc-ELISA) tested, a protocol using either anti-saxitoxin (STX)/STX-horseradish peroxidase (HRP) or anti-neo-STX/STX-HRP pairs was found to be most effective for screening paralytic shellfish poisoning (PSP) toxins in contaminated shellfish. An excellent agreement between the total PSP toxins (STX plus neo-STX levels) obtained by ELISA and by mouse assay was found. Analysis of 1540 naturally contaminated samples revealed that 211 (13.6%) samples were positive by the ELISA as compared with 175 (11.3%) by the mouse assays at the threshold level of 80 mu g/100 g of sample. The distributions of the toxins at levels >40 and >80 mu g/100 g in 1540 samples obtained by different ELISA formats are presented. The total PSP toxin levels (STX plus neo-STX) obtained from simultaneous analysis of both STX and neo-STX by ELISA are valid for accurate screening for the presence of PSP toxins in these samples and could eliminate as much as 80-85% of the samples needed to run a mouse assay.