Listeria's right-handed helical rocket-tail trajectories: mechanistic implications for force generation in actin-based motility.
Listeria's right-handed helical rocket-tail trajectories: mechanistic implications for force generation in actin-based motility.
复制标题
李斯特菌的右手螺旋火箭尾轨迹：基于肌动蛋白的运动力产生的机械意义。
DOI：
10.1002/cm.20050
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Purich,DanielL
中科院分区：
文献类型：
--
作者：
Zeile,WilliamL;Zhang,Fangliang;Dickinson,RichardB;Purich,DanielL
单核细胞增生李斯特菌在无细胞提取物中基于肌动蛋白的运动过程中形成右手螺旋火箭尾轨迹，并且这种立体化学特征与actoclampin的亲和力调节的夹紧丝伸长模型一致[Dickinson and Purich，2002：Biophys J82：605-617]。在该机制中，右旋扭矩由末端跟踪分子马达产生，每个马达由细丝倒刺末端和夹持蛋白组成，夹持蛋白可追踪其细丝伴侣的右旋螺旋。相比之下，扭矩不是那些模型的预测属性（例如，弹性推进、弹性布朗棘轮、栓系棘轮和插入聚合模型），要求长丝倒刺末端在每个单体添加步骤期间/之后从运动物体的表面离开/分离。螺旋轨迹也解释了为什么李斯特菌在长度尺度上经历纵向轴旋转，与李斯特菌火箭尾巴的螺旋周期相匹配。细胞动力。Cytoskeleton 60：121-128，2005.© 2004 Wiley利斯公司
Listeria monocytogenesforms right‐handed helical rocket tail trajectories during actin‐based motility in cell‐free extracts, and this stereochemical feature is consistent with actoclampin's affinity‐modulated, clamped‐filament elongation model [Dickinson and Purich, 2002:Biophys J82:605–617]. In that mechanism, right‐handed torque is generated by an end‐tracking molecular motor, each comprised of a filament barbed end and clamping protein that processively traces the right‐handed helix of its filament partner. By contrast, torque is not a predicted property of those models (e.g., elastic propulsion, elastic Brownian ratchet, tethered ratchet, and insertional polymerization models) requiring filament barbed ends to depart/detach from the motile object's surface during/after each monomer‐addition step. Helical trajectories also explain whyListeriaundergoes longitudinal‐axis rotation on a length‐scale matching the helical periodicity ofListeria's rocket tails. Cell Motil. Cytoskeleton 60:121–128, 2005. © 2004 Wiley‐Liss, Inc.