Optimum Conditions of Low Light Irradiation-CA Storage for Quality Preservation of Grafted Tomato Plug Seedlings
Optimum Conditions of Low Light Irradiation-CA Storage for Quality Preservation of Grafted Tomato Plug Seedlings
复制标题
番茄嫁接穴盘苗微光辐照CA贮藏保质的最佳条件
DOI：
10.2525/ecb1963.39.111
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Iimoto
中科院分区：
文献类型：
--
作者：
K. Fujiwara;S. Isobe;M. Iimoto
通过两个试验，确定了CO2浓度和光合光量子通量密度(PPFD)在大气O2浓度下弱光控制气调(LLI-CA)贮藏条件下对嫁接番茄品质保鲜的最佳组合。砧木：cv.帮手M)插苗。在第一个实验中，将幼苗置于10℃的玻璃容器中，测量了不同CO2浓度(0.05、0.5或1.0%)和PPFDs(0、1.0、2.0和3.0pmolm-2 S-1)下发光二极管发出的红光下的CO2交换速率。绘制了CO2浓度、PPFD和CO2交换率的三维曲线图，得到了CO2浓度与PPFD之间的关系曲线，其中CO2交换率为0。在理论上，CO2浓度和PPFD曲线上的所有组合都是在贮藏过程中保持苗木干重接近恒定的最基本的条件。在第二个试验中，嫁接番茄穴盘苗在10℃下贮藏28d，试验条件为：0.05%+2.5μmolm-2 S(C0.05P2.5)，0.25%+1.9μmolm-2 S-1(C0.25P1.9)，0.50%+1.3μmolm-2 S-1(CO.SOPL3)，0.75%+0.9μ摩尔m-2 S(C0.75P0.9)和1.0%+0.5μ摩尔m-2 S(C1.0P0.5)。以0.05%CO_2+0μ摩尔m~(-2)、S~(-1)PPFD的贮藏条件为对照。在贮藏28d的最后一天，苗木的五级主观视觉质量得分和叶片色差值(CONT EAB)的顺序分别为：C0.25P1.9>；C0.50Pl.3>；C0.05P2.5=C0.75P0.9>；C1.0P0.5>；Cont和Δ=C1.0P0.5=C0.75P0.9=C0.05P2.5>；C0.50P1.3>；C0.25P1.9(cont>；C0.05P2.5)(式中‘>；‘和’=‘分别表示在LSD的5%水平上显著大于和没有显著差异)。在贮藏28d的最后一天，C0.25P1.9和C0.50P1.3的成活率为100%(10株/10株)，而C0.05P2.5、C0.75P0.9、C1.0P0.5和Cont的成活率分别为90%、70%、20%和0%。结果表明，在大气O2浓度下，嫁接番茄穴盘苗在10℃下进行LL-CA贮藏的最佳条件为0.25%CO2+1.9ymolm~(-2)S-1PPFD。这些结果还表明，成功地进行LL-CA贮藏存在一个最低PPFD水平，所用苗木的最低PPFD水平被认为是0.9umol m-2 S-1之间。
Two experiments were carried out to determine the optimum combination of CO2 concentration and photosynthetic photon flux density (PPFD) under low light irradiation controlled atmosphere (LLI-CA) storage conditions at atmospheric O2 concentration for the quality preservation of grafted tomato (Lycopersicon esculentum Mill., scion : cv. Momotaro ; rootstock : cv. Helper M) plug seedlings. In the first experiment, the seedlings were placed in a glass vessel at 10°C, and CO2 exchange rate was measured under different CO2 concentrations (0.05, 0.5 or 1.0%) and PPFDs (0, 1.0, 2.0 or 3.0pmol m-2 s-1) of red light from light-emitting diodes. A 3-dimensional (CO2 concentration, PPFD and CO2 exchange rate) graph was drawn to find a curve expressing the relationship between CO2 concentration and PPFD where the CO2 exchange rate becomes 0. All combinations of CO2 concentration and PPFD on the curve are theoretically the most essential conditions for keeping dry weight of the seedlings nearly constant during storage. In the second experiment, grafted tomato plug seedlings were stored at 10°C for 28 d under the following combinations of CO2 concentration and PPFD selected from the curve : 0.05% + 2.5μmol m-2 s-1 (C0.05P2.5), 0.25% + 1.9 μmol m-2 s-1 (C0.25P1.9), 0.50% + 1.3 μmol m-2 s-1 (CO.SOPL3), 0.75% + 0.9μmol m-2 s-1 (C0.75P0.9) and 1.0% + 0.5 μmol m-2 s-1 (C1.0P0.5) . Storage conditions of 0.05% CO2+0μmol m-2 s-1 PPFD were used as the control (font) . Five-rank subjective visual quality scores of the seedlings and color difference values of the leaves (ΔEab) on the last day of 28 d storage were in the following order: C0.25P1.9>C0.50Pl.3>C0.05P2.5=C0.75P0.9>C1.0P0.5>Cont and Cont = C1.0P0.5 = C0.75P0.9 = C0.05P2.5>C0.50P1.3>C0.25P1.9 (Cont> C0.05P2.5), respectively (where‘>’ and ‘=’ denote significantly greater and not significantly different at the 5% level by LSD, respectively) . Survival percentages in C0.25P 1.9 and C0.50P1.3 were 100% (10 plants out of 10) on the last day of 28 d cultivation following 28 d storage whereas survival percentages in C0.05P2.5, C0.75P0.9, C 1.0P0.5 and Cont were 90, 70, 20 and 0%, respectively. These results showed that the optimum conditions of LL-CA storage for quality preservation of grafted tomato plug seedlings at l0°C under atmospheric O2 concentration were 0.25% CO2 combined with 1.9ymol m-2 s-1 PPFD. These results also indicate that there exists a minimum level of PPFD for successful LL-CA storage, and the minimum was considered to be between 0.9 and 1.3, umol m-2 s-1 for the seedlings used.