INTERACTION OF ALUI, CFR6I AND PVUII RESTRICTION-MODIFICATION ENZYMES WITH SUBSTRATES CONTAINING EITHER N4-METHYLCYTOSINE OR 5-METHYLCYTOSINE
INTERACTION OF ALUI, CFR6I AND PVUII RESTRICTION-MODIFICATION ENZYMES WITH SUBSTRATES CONTAINING EITHER N4-METHYLCYTOSINE OR 5-METHYLCYTOSINE
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DOI：
10.1016/0167-4781(87)90078-9
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发表时间：
1987-08-25
期刊：
影响因子：
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通讯作者：
JANULAITIS, A
中科院分区：
文献类型：
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作者：
BUTKUS, V;KLIMASAUSKAS, S;JANULAITIS, A
R.Cfr61（PvuII 限制性核酸内切酶的同裂酶）的切割特异性确定为 5''CAG.dwnarw.CTG，Cfr-6I 和 PvuII 甲基化酶的甲基化特异性确定为 5''CAG4mCTG。因此，M.Cfr6I 和 M.PvuII 是具有 N4-甲基胞嘧啶特异性的甲基化酶列表中的新成员。上述 RM 酶均不作用于同源甲基化位置含有 N4-甲基胞嘧啶或 5-甲基胞嘧啶的底物。
The cleavage specificity of R.Cfr61, an isoschizomer of PvuII restriction endonuclease was determined to be 5''CAG.dwnarw.CTG and the methylation specificity of Cfr-6I and PvuII methylases, 5''CAG4mCTG. Thus, M.Cfr6I and M.PvuII are new additions to the list of methylases with N4-methylcytosine specificity. Neither of the above RM enzymes acts on the substrates containing either N4-methylcytosine or 5-methylcytosine in a cognate methylation position.