Fusion imaging of fluorescent and phase-contrast x-ray computed tomography using synchrotron radiation in medical biology
Fusion imaging of fluorescent and phase-contrast x-ray computed tomography using synchrotron radiation in medical biology
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DOI：
10.1117/12.680133
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发表时间：
2006-08
期刊：
影响因子：
64.8
通讯作者：
Jin Wu;T. Takeda;T. Lwin;N. Sunaguchi;T. Fukami;T. Yuasa;M. Minami;T. Akatsuka
中科院分区：
文献类型：
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作者：
Jin Wu;T. Takeda;T. Lwin;N. Sunaguchi;T. Fukami;T. Yuasa;M. Minami;T. Akatsuka
我们集成了荧光X射线计算机断层扫描(FXCT)和相位对比X射线计算机断层扫描(PCCT)，并评估了这种融合成像在小动物中的可行性。建立小鼠脑肿瘤模型和金黄地鼠心肌病模型。静脉注射脑灌注剂127I-IMP和心肌脂肪酸代谢剂127I-BMIPP后分别取脑和心脏。各靶器官用福尔马林固定，用于FXCT和PCCT。获得三维(3D)图像，并对3D图像进行重采样，确定脑和心脏的表面轮廓，以便以相同的空间分辨率进行描述。通过3D图像处理软件将这些图像交互地融合到显示的图像上。FXCT分别以0.5 mm和0.2 mm的空间分辨率清晰显示IMP脑灌注像和BMIPP脂肪酸代谢像。0.03 mm空间分辨率的PCCT图像清晰地显示了大脑皮质、海马体、侧脑室、小脑等形态结构，以及心腔、乳头肌、左右室等心脏的形态结构。在融合图像上，容易识别脑血流灌注和心肌脂肪酸代谢异常的位置和程度。我们的结果表明，FXCT和PCCT的整合对于了解与其解剖定位相对应的生物状态非常有用，即使在小动物中也是如此。
We integrated fluorescent X-ray computed tomography (FXCT) and phase-contrast X-ray computed tomography (PCCT), and the feasibility of this fusion imaging was assessed for small animals. Brain tumor model of mouse and cardiomyopathic model of hamsters were examined. The brain and heart were extracted after intravenous injection of cerebral perfusion agent 127I-IMP and myocardial fatty acid metabolic agent 127I-BMIPP, respectively. Each target organ was fixed by formalin for FXCT and PCCT. Images were obtained three-dimensionally (3D), and the surface contour of brain and heart were determined from 3D-image after re-sampling for the description with the same spatial resolution. These images were fused interactively on displayed images by 3D image manipulation software. In FXCT, cerebral perfusion image with IMP and fatty acid metabolic image with BMIPP were clearly demonstrated at 0.5 mm and 0.2 mm spatial resolution, respectively. PCCT image with 0.03 mm spatial resolution depicted clearly the morphological structures of brain such as cerebral cortex, hippocampus, lateral ventricle and cerebellum, and for heart such as cardiac lumen, papillary muscle, left and right ventricle. On fusion image, localization and degree of abnormality of cerebral perfusion and myocardial fatty acid metabolism were easily recognized. Our results suggested that the integration of FXCT and PCCT is very useful to understand biological state corresponding to its anatomical localization even in small animal.