Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules
Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules
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DOI：
10.1021/ol702920e
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发表时间：
2008-04-17
期刊：
影响因子：
5.2
通讯作者：
Armitage, Bruce A.
中科院分区：
文献类型：
--
作者：
Constantin, Tudor P.;Silva, Gloria L.;Armitage, Bruce A.
合成了一种新的荧光花青染料，发现其在流体溶液中以及存在双链DNA时荧光量子产率较低，但在粘性甘油溶液中荧光增强了80倍。一种被筛选用于与这种新染料结合的RNA适体表现出K - d = 87 nM以及60倍的荧光增强。染料 - 适体对是一种荧光模块，可被纳入荧光传感器和标记物中。
A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K-d = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.