Salmonid alphavirus infection causes skin dysbiosis in Atlantic salmon (Salmo salar L.) post-smolts.
Salmonid alphavirus infection causes skin dysbiosis in Atlantic salmon (Salmo salar L.) post-smolts.
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DOI：
10.1371/journal.pone.0172856
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Salinas I
中科院分区：
文献类型：
--
作者：
Reid KM;Patel S;Robinson AJ;Bu L;Jarungsriapisit J;Moore LJ;Salinas I
宿主、微生物区系和病毒病原体之间的相互作用是复杂的，人们对此知之甚少。本研究旨在探讨大西洋鲑鱼(Salmo salar L.)皮肤微生物群落的变化。以应对实验性感染沙门氏甲型病毒(SAV)。用16S rDNA焦磷酸测序技术测定了对照组、低剂量SAV感染后7天、低剂量SAV感染后14天、高剂量SAV感染后7天和高剂量SAV感染后14天的鲑鱼皮肤微生物群落。感染治疗和感染后的时间都是皮肤微生物群落组成的强烈预测因子。受SAV3感染的鱼的皮肤样本显示出不平衡的微生物区系，其特征是变形杆菌的数量减少，如Oleispira sp.。机会性类群增加，包括黄杆菌科、链球菌科和扁杆菌属。这些结果表明，病毒感染会导致皮肤生物失调，可能会使宿主更容易受到继发性细菌感染。
Interactions among host, microbiota and viral pathogens are complex and poorly understood. The goal of the present study is to assess the changes in the skin microbial community of Atlantic salmon (Salmo salar L.) in response to experimental infection with salmonid alphavirus (SAV). The salmon skin microbial community was determined using 16S rDNA pyrosequencing in five different experimental groups: control, 7 days after infection with low-dose SAV, 14 days after infection with low-dose SAV, 7 days after infection with high-dose SAV, and 14 days after infection with high-dose SAV. Both infection treatment and time after infection were strong predictors of the skin microbial community composition. Skin samples from SAV3 infected fish showed an unbalanced microbiota characterized by a decreased abundance of Proteobacteria such as Oleispira sp. and increased abundances of opportunistic taxa including Flavobacteriaceae, Streptococcaceae and Tenacibaculum sp. These results demonstrate that viral infections can result in skin dysbiosis likely rendering the host more susceptible to secondary bacterial infections.