TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma
TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma
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DOI：
10.3892/mmr.2016.5183
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发表时间：
2016-06-01
影响因子：
3.4
通讯作者：
Li, Shugang
中科院分区：
文献类型：
--
作者：
Liang, Jinqian;Xing, Dan;Li, Shugang
骨肉瘤是一种常见的癌症，具有很高的转移能力，特别是转移到肺部。有效的治疗策略已经得到改善，然而，这种恶性肿瘤发病的详细分子机制仍有待充分阐明。目前的研究调查了三联基序（TRIM）家族蛋白 TRIM59 在骨肉瘤生长和转移中的作用。研究发现TRIM59在临床骨肉瘤组织和培养的骨肉瘤细胞系中过表达。此外，MTT实验表明，在U2OS和MG63细胞中，特异性siRNA敲低TRIM59可抑制细胞增殖，而TRIM59过表达可促进细胞增殖。此外，在 Transwell 小室检测中，TRIM59 的过表达显着增加了 U2OS 细胞的迁移和侵袭能力。此外，TRIM59能够负向调节P53的蛋白水平，而不显着影响U2OS和MG63细胞中的mRNA水平。这些数据表明 TRIM59 在骨肉瘤中具有致癌能力，可促进骨肉瘤细胞增殖、迁移和侵袭。
Osteosarcoma is a prevalent type of cancer and has a high metastatic ability, particularly for metastasis to the lungs. Effective treatment strategies have improved, however, the detailed molecular mechanism underlying the onset of this malignancy remains to be fully elucidated. The current study investigated the role of the tripartite motif (TRIM) family protein TRIM59 in osteosarcoma growth and metastasis. It was identified that TRIM59 was overexpressed in clinical osteosarcoma tissues and cultured osteosarcoma cell lines. In addition, the MTT assay demonstrated that in U2OS and MG63 cells, knockdown of TRIM59 by specific siRNA inhibited proliferation, whereas overexpression of TRIM59 promoted cell proliferation. Furthermore, overexpression of TRIM59 significantly increased the U2OS cell migrative and invasive abilities in a Transwell chamber assay. In addition, TRIM59 was able to negatively regulate the protein levels of P53 without significantly affecting the mRNA levels in U2OS and MG63 cells. These data suggest the oncogenic abilities of TRIM59 in osteosarcoma, which promote osteosarcoma cell proliferation, migration and invasion.