Erosion of efficiency in non‐uniform linear chromatography
Erosion of efficiency in non‐uniform linear chromatography
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非均匀线性色谱效率的下降
DOI：
10.1002/jhrc.1240160106
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
L. Blumberg
中科院分区：
文献类型：
--
作者：
L. Blumberg
在线性层析(即在没有样品过载的情况下进行的层析)中，当柱的板高度沿其长度大致均匀时，溶质速度的变化是唯一可能导致效率下降的原因。这些变化的来源(容量比和流动相密度的变化等)除了对溶质速度的影响外，对效率的侵蚀没有直接作用。换句话说，最终导致效率下降的仅仅是溶质穿过柱子中同样小的部分所需时间的变化。严重的侵蚀只有在柱的一个或几个相对较小的部分中的溶质突然和深度减速时才会发生。
如果要使线性SFC中的压力梯度对效率的侵蚀很大，则溶质减速的深度和程度必须超过迄今实验所证实的深度和程度。对许多相关实例进行了图解分析。
In linear chromatography (i.e. chromatography performed in the absence of sample overloading), when the plate height of a column is roughly uniform along its length, variations in the velocities of solutes are the only possible causes of erosion of efficiency. The sources of these variations (variations in capacity ratio and in the density of the mobile phase, etc.) play no direct role in the erosion of efficiency except through their effect on solute velocities. In other words, what eventually causes the erosion of efficiency is merely variation in the time required for solutes to traverse equally small segments of a column. Significant erosion can only arise from abrupt and deep deceleration of solutes in one or several relatively small segments of a column.
If erosion of efficiency caused by pressure gradients in linear SFC is to be large, the depth and the sharpness of the deceleration of a solute must go beyond that hitherto confirmed experimentally. Many relevant examples are analyzed graphically.