The energy profiling of short interfering RNAs is highly predictive of their activity
The energy profiling of short interfering RNAs is highly predictive of their activity
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DOI：
10.1089/1545457042258260
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Rainaldi, G
中科院分区：
文献类型：
--
作者：
Poliseno, L;Evangelista, M;Rainaldi, G
通过合成短干扰rna （sirna）来敲低基因表达的技术由于其相对简单和可靠而得到广泛应用。尽管声称活性sirna很容易被识别，但如何高效地选择活性sirna并没有完全明确。在这里，我们报告能量分析可以用于siRNA设计的指导方针。
The technology of knockdown of gene expression by synthetic short interfering RNAs (siRNAs) is currently diffused because of its relative simplicity and reliability. Despite the claims that active siRNAs can be easily identified, how to select active siRNAs with high efficiency it is not fully defined. Here, we report that energy profiling can be used in the guidelines for siRNA design.