Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in Accessions of Arabidopsis
Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in Accessions of Arabidopsis
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DOI：
10.1094/mpmi-02-10-0047
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发表时间：
2010-12-01
影响因子：
3.5
通讯作者：
Grant, Murray R.
中科院分区：
文献类型：
--
作者：
Forsyth, Alec;Mansfield, John W.;Grant, Murray R.
我们已经研究了拟南芥中非寄主抗性的遗传学，使用菜豆病原体假单胞菌菜豆致病变种小种6 1448 A来探测基础防御的自然变异。Niedersenz（Nd）的叶子中很少出现症状，哥伦比亚（Col）出现一些黄化和偶尔坏死，而Wassilewskija（Ws）在接种后通过渗入观察到组织塌陷。从Col和Nd的杂交揭示了一种连续症状增加的模式，表明抗性的数量决定因素的操作。通过定位数量性状位点（QTL），确定了抗性的显著连锁（低症状评分）对4号染色体上标记的标记在Nd × Ws的F2中分离表明两个显性抗性基因的操作，其中之一是编码鞭毛蛋白受体的FLS2。通过转基因实验证实了需要FLS2来赋予抗性，并且我们表明对菜豆疫霉致病变种的应答受FLS2基因剂量的影响。使用RIL，FLS2和第1染色体上的QTL都是Nd对细菌定殖和症状发展的最高抗性水平所必需的
We have examined the genetics of nonhost resistance in Arabidopsis, using the bean pathogen Pseudomonas syringae pv phaseolicola race 6 1448A to probe accessions for natural variation in basal defense Symptoms rarely developed in leaves of Niedersenz (Nd), some yellowing and occasional necrosis developed in Columbia (Col), whereas tissue collapse was observed in Wassilewskija (Ws) after inoculation by infiltration Analysis of F2 progeny and recombinant Inbred lines (RIL) from a cross between Col and Nd revealed a pattern of continuous symptom increase, indicating the operation of quantitative determinants of resistance By mapping quantitative trait loci (QTL), significant linkage was determined for resistance (low symptom score) to markers on chromosome 4 Segregation in the F2 cross from Nd x Ws indicated the operation of two dominant genes for resistance, one of which was FLS2 encoding the flagellin receptor The requirement for FLS2 to confer resistance was confirmed by transgenic experiments, and we showed that the response to P syringae pv phaseohcola was affected by FLS2 gene dosage Using RIL, the second locus was mapped as a QTL to a large Interval on chromosome 1 Both FLS2 and the QTL on chromosome 1 were required for the highest level of resistance to bacterial colonization and symptom development in Nd