Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation
Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation
复制标题
海参夏眠呼吸树中miRNA表达谱的大规模鉴定及比较分析
DOI：
10.1016/j.margen.2014.01.002
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发表时间：
2014-02-01
期刊：
影响因子：
1.9
通讯作者：
Storey, Kenneth B.
中科院分区：
文献类型：
--
作者：
Chen, Muyan;Storey, Kenneth B.
The sea cucumber Apostichopus japonicus withstands high water temperatures in the summer by suppressing its metabolic rate and entering a state of aestivation. We hypothesized that changes in the expression of miRNAs could provide important post-transcriptional regulation of gene expression during hypometabolism via control over mRNA translation. The present study analyzed profiles of miRNA expression in the sea cucumber respiratory tree using Solexa deep sequencing technology. We identified 279 sea cucumber miRNAs, including 15 novel miRNAs specific to sea cucumber. Animals sampled during deep aestivation (DA; after at least 15 days of continuous torpor) were compared with animals from a non-aestivation (NA) state (animals that had passed through aestivation and returned to an active state). We identified 30 differentially expressed miRNAs ([RPM (reads per million) > 10,)vertical bar FC vertical bar (vertical bar fold change vertical bar) >= 1, FDR (false discovery rate)
