REGIONAL CHANGES IN CEREBRAL HEMODYNAMICS AS A RESULT OF A VISUAL STIMULUS MEASURED BY NEAR-INFRARED SPECTROSCOPY
REGIONAL CHANGES IN CEREBRAL HEMODYNAMICS AS A RESULT OF A VISUAL STIMULUS MEASURED BY NEAR-INFRARED SPECTROSCOPY
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DOI：
10.1098/rspb.1995.0158
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发表时间：
1995-09-22
影响因子：
4.7
通讯作者：
ZEKI, S
中科院分区：
文献类型：
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作者：
MEEK, JH;ELWELL, CE;ZEKI, S
近红外光谱（NIRS）用于测量脑血流动力学的整体变化。我们已经采用该技术来测量响应视觉刺激的区域变化。十名志愿者接受了计算机生成的视觉刺激，旨在激活大面积的视觉皮层，包括 V1、V2、V3、V4 和 V5。刺激开启 30 秒，关闭 30 秒。使用商用光谱仪 (NIRO500) 测量枕叶皮质上的氧合血红蛋白 ([HbO(2)]) 和脱氧血红蛋白 ([Hb]) 浓度的变化。将十个周期的数据相加。作为对照，在额叶皮层上重复了该实验。对于每个受试者，[HbO(2)] 在刺激期间增加，在刺激关闭时减少。 [HbO(2)] 的平均 (+/-s.e.m.) 变化为 0.54 +/- 0.14 mu mol l(-1)。由[HbO(2)] + [Hb]给出的总血红蛋白浓度的变化为0.61+/-0.21μmol·l(-1)，相当于脑血容量增加0.04+/-0.01ml·100g(-1)，约占总脑血容量的2%。额叶皮质的[HbO(2)]没有显着变化，这意味着血容量的变化源自枕叶。这表明 NIRS 提供了一种非侵入性方法来测量视觉刺激导致的脑血流动力学区域变化。
Near infrared spectroscopy (NIRS) is used to measure global changes in cerebral haemodynamics. We have adapted the technique to measure regional changes in response to a visual stimulus. Ten volunteers were exposed to a computer generated visual stimulus designed to activate a large area of the visual cortex, including V1, V2, V3, V4 and V5. The stimulus was on for 30 s and off for 30 s. Changes in the concentrations of oxyhaemoglobin ([HbO(2)]) and deoxyhaemoglobin ([Hb]) were measured using a commercial spectrometer (NIRO500), over the occipital cortex. The data were summed over ten cycles. As a control, the experiment was repeated over the frontal cortex. For each subject [HbO(2)] increased during stimulation, and decreased when the stimulus was off. The mean (+/-s.e.m.) change in [HbO(2)] was 0.54 +/- 0.14 mu mol l(-1). The change in total haemoglobin concentration, given by [HbO(2)] + [Hb] was 0.61 +/- 0.21 mu mol l(-1), equivalent to a rise in cerebral blood volume of 0.04 +/- 0.01 ml 100 g(-1) which is about 2% of the total cerebral blood volume. There was no significant change in [HbO(2)] over the frontal cortex, implying that the changes in blood volume originated in the occipital lobe. This demonstrates that NIRS provides a non-invasive method of measuring regional changes in cerebral haemodynamics as a result of visual stimulation.