Cryptic Aspergillus nidulans Antimicrobials
Cryptic Aspergillus nidulans Antimicrobials
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DOI：
10.1128/aem.02000-10
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发表时间：
2011-06-01
影响因子：
4.4
通讯作者：
Keller, Nancy P.
中科院分区：
文献类型：
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作者：
Giles, Steve S.;Soukup, Alexandra A.;Keller, Nancy P.
真菌的次级代谢产物（SM）的生产被假设为生产生物体提供一些健身属性。然而，大多数SM簇是“沉默”的真菌在传统的实验室环境中生长时，很难确定这些SM簇产品的任何功能或活性。最近，在构巢曲霉中产生的染色质重塑突变体诱导了几个隐藏的SM基因簇的激活。从这个突变体的9个纯化的代谢产物的系统测试确定了大黄素衍生物对人类真菌病原体（抑制孢子萌发和菌丝生长）和几种细菌的功效。过氧化氢酶降低这种抗微生物活性的能力暗示了活性氧物质的产生，特别是过氧化氢的产生，作为大黄素羟基活性的机制。
Secondary metabolite (SM) production by fungi is hypothesized to provide some fitness attribute for the producing organisms. However, most SM clusters are "silent" when fungi are grown in traditional laboratory settings, and it is difficult to ascertain any function or activity of these SM cluster products. Recently, the creation of a chromatin remodeling mutant in Aspergillus nidulans induced activation of several cryptic SM gene clusters. Systematic testing of nine purified metabolites from this mutant identified an emodin derivate with efficacy against both human fungal pathogens (inhibiting both spore germination and hyphal growth) and several bacteria. The ability of catalase to diminish this antimicrobial activity implicates reactive oxygen species generation, specifically, the generation of hydrogen peroxide, as the mechanism of emodin hydroxyl activity.