Doublecortin-expressing cells persist in the associative cerebral cortex and amygdala in aged nonhuman primates
Doublecortin-expressing cells persist in the associative cerebral cortex and amygdala in aged nonhuman primates
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DOI：
10.3389/neuro.05.017.2009
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发表时间：
2009-10-13
影响因子：
2.9
通讯作者：
Yan, Xiao-Xin
中科院分区：
文献类型：
--
作者：
Zhang, Xue-Mei;Cai, Yan;Yan, Xiao-Xin
最近在相对较大的哺乳动物（豚鼠、兔、猫、猴和人）的整个成年大脑皮层中鉴定出一种表达典型的未成熟神经元标记物（包括双皮质素（DCX+））的新型细胞群。这些细胞在相对于初级皮质区域的联合中更常见，并且似乎发育成中间神经元，包括II型氮能神经元。在这里，我们进一步描述了这些细胞在大脑皮层和杏仁核，在海马齿状回的DCX+细胞相比，在三个年龄组的恒河猴：青年（12.3 +/- 0.2岁，n = 3），中年（21.2 +/- 1.9岁，n = 3）和老年（31.3 +/- 1.8岁，n = 4）。具有异质形态的DCX+细胞持续存在于所有组中主要在联合皮质和杏仁核上的第II/III层中（包括具有脑淀粉样蛋白病理学的两只老年动物），显示出细胞密度随年龄的平行下降。与皮质和杏仁核相反，颗粒下区的DCX+细胞在中年和老年组中减少。DCX+皮质细胞在中、老年组可呈切线形长迁移链排列，老年组细胞团明显扭曲。皮质DCX+细胞通常与聚唾液酸化神经细胞粘附分子共定位，部分与神经元特异性核蛋白共定位，氨基丁酸，表明这些细胞分化成中间神经元表型的潜力。这些数据表明，在非人类灵长类动物的大脑皮层和杏仁核中，未成熟的中间神经元样细胞具有终身的作用。
A novel population of cells that express typical immature neuronal markers including doublecortin (DCX+) has been recently identified throughout the adult cerebral cortex of relatively large mammals (guinea pig, rabbit, cat, monkey and human). These cells are more common in the associative relative to primary cortical areas and appear to develop into interneurons including type II nitrinergic neurons. Here we further describe these cells in the cerebral cortex and amygdala, in comparison with DCX+ cells in the hippocampal dentate gyrus, in three age groups of rhesus monkeys: young adult (12.3 +/- 0.2 years, n = 3), mid-age (21.2 +/- 1.9 years, n = 3) and aged (31.3 +/- 1.8 years, n = 4). DCX+ cells with a heterogeneous morphology persisted in layers II/III primarily over the associative cortex and amygdala in all groups (including in two old animals with cerebral amyloid pathology), showing a parallel decline in cell density with age across regions. In contrast to the cortex and amygdala, DCX+ cells in the subgranular zone diminished in the mid-age and aged groups. DCX+ cortical cells might arrange as long tangential migratory chains in the mid-age and aged animals, with apparently distorted cell clusters seen in the aged group. Cortical DCX+ cells colocalized commonly with polysialylated neural cell adhesion molecule and partially with neuron-specific nuclear protein and.-aminobutyric acid, suggesting a potential differentiation of these cells into interneuron phenotype. These data suggest a life-long role for immature interneuron-like cells in the associative cerebral cortex and amygdala in nonhuman primates.