Ectopic bone formation in rat marrow stromal cell/titanium fiber mesh scaffold constructs: Effect of initial cell phenotype
Ectopic bone formation in rat marrow stromal cell/titanium fiber mesh scaffold constructs: Effect of initial cell phenotype
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DOI：
10.1016/j.biomaterials.2005.04.006
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发表时间：
2005-11-01
期刊：
影响因子：
14
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Holtorf, HL;Jansen, JA;Mikos, AG
钛纤维网支架已被证明是一种适合用于培养原代骨髓基质细胞的材料，旨在构建用于骨组织替代的组织工程结构。在天然骨组织中，已知这些细胞通过整合素受体与细胞外基质分子结合，这些受体针对特定的肽序列，并且这些结合可以是细胞信号传导的来源，影响细胞的分化等行为。在本研究中，我们检测了处于成骨细胞分化两个不同阶段的原代大鼠骨髓基质细胞在接种到表面连接或未连接RGD肽的钛纤维网支架上时，在体外和体内进一步分化为成骨细胞的能力。在体外，连接的RGD肽导致初始细胞增殖减少。在体内，在大鼠皮下植入模型中，连接的RGD肽对异位骨形成没有影响。在植入前暴露于地塞米松4天的支架/细胞结构（+地塞米松结构）导致显著的骨形成，而在 - 地塞米松结构中未观察到骨形成。这些结果表明，骨髓基质细胞的成骨细胞分化不依赖于表面连接的RGD肽，并且植入细胞的初始分化阶段在钛纤维网骨组织工程结构的骨形成中起重要作用。（c）2005爱思唯尔有限公司。保留所有权利。
Titanium fiber mesh scaffolds have been shown to be a suitable material for culture of primary marrow stromal cells in an effort to create tissue engineered constructs for bone tissue replacement. In native bone tissue, these cells are known to attach to extracellular matrix molecules via integrin receptors for specific peptide sequences, and these attachments can be a source of cell signaling, affecting cell behaviors such as differentiation. In this study, we examined the ability of primary rat marrow stromal cells at two different stages of osteoblastic differentiation to further differentiate into osteoblasts both in vitro and in vivo when seeded on titanium fiber mesh scaffolds either with or without RGD peptide tethered to the surface. In vitro, the tethered RGD peptide resulted in reduced initial cell proliferation. In vivo, there was no effect of tethered RGD peptide on ectopic bone formation in a rat subcutaneous implant model. Scaffold/cell constructs exposed to dexamethasone for 4 days prior to implantation (+dex constructs) resulted in significant bone formation whereas no bone formation was observed in -dex constructs. These results show that the osteoblastic differentiation of marrow stromal cells was not dependent on surface tethered RGD peptide, and that the initial differentiation stage of implanted cells plays an important role in bone formation in titanium fiber mesh bone tissue engineering constructs. (c) 2005 Elsevier Ltd. All rights reserved.