POSTNATAL DIFFERENTIATION AND DEVELOPMENT OF THE RAT EPIDIDYMIS - A STEREOLOGICAL STUDY
POSTNATAL DIFFERENTIATION AND DEVELOPMENT OF THE RAT EPIDIDYMIS - A STEREOLOGICAL STUDY
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DOI：
10.1002/ar.1092380205
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发表时间：
1994-02-01
影响因子：
--
通讯作者：
WREFORD, NG
中科院分区：
文献类型：
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作者：
JIANG, FX;TEMPLESMITH, P;WREFORD, NG
用体视学方法研究了15 ~ 120天大鼠附睾的发育和分化。测定附睾初始节段、头、体和尾的上皮、间质和管腔室的相对体积（体积密度）和绝对体积。在所有节段中，上皮腔室的体积密度在第15至30天之间均有所增加，然后在第45天（0.476 +/- 0.031）、第60天（0.258 +/- 0.028）、第90天（0.245 +/- 0.007）和尾节（0.140 +/- 0.004）降至成虫值。间质的相对体积减小，而管腔的相对体积在同一时期增加，近段比远段更早达到成人值。与体积分数相比，从第15天到第90天，所有节段的所有隔室的绝对体积都增加了。90 ~ 120 d间，初始节段和头节的绝对体积变化不大。在同一时期内，脑体和尾区的所有隔室的体积均显著增加。在上皮的管腔和基底膜方面的表面积上观察到类似的发育模式；所有节段的单位体积表面积（表面密度）在大约60天时达到成虫值，而初始节段和头节的绝对表面面积的增加在90天时停止，而在体部和尾部继续增加到120天。90 d后附睾小管总长度变化规律相同，初段和头段长度无明显增加。然而，在90至120天期间，体尾长度继续增加。小管和管腔直径在第60天达到初始节段的确定值，但在第90天远节段继续增加。上皮高度在15 ~ 30 d间呈上升趋势；头部上皮高度在第30天最高，随后略有下降；体部和尾部最大上皮高度在第45天分别下降到第60天和第90天的稳定值。所有的数据都与附睾的近端分化和远端发育一致，在确定的结构建立后，所有节段的生长都在继续。在近端节段停止生长后，远端节段继续生长。(C) 1994 Wiley-Liss, Inc。
Postnatal development and differentiation of the rat epididymis was studied in the rat from 15 to 120 days of life using stereological techniques. Both the relative volume (volume density) and absolute volume of the epithelial, interstitial, and luminal compartments in the initial segment, caput, corpus, and cauda epididymides were determined. In all segments the volume density of the epithelial compartment increased between days 15 and 30 before falling to adult values at 45 days in the initial segment (0.476 +/- 0.031), at 60 days in the caput (0.258 +/- 0.028) and at 90 days in the corpus (0.245 +/- 0.007) and cauda (0.140 +/- 0.004). The relative volume of the interstitium decreased, whilst that of the lumen increased over the same period with adult values being achieved earlier in the proximal segments than in the distal segments. In contrast to volume fraction the absolute volume of all compartments in all segments increased from day 15 to day 90. Between 90 and 120 days the absolute volumes of compartments in the initial segment and caput showed little volume change. All compartments in the corpus and cauda showed significant increases in volume over the same period. A similar pattern of development was observed with respect to the surface area of both the luminal and basement membrane aspects of the epithelium; surface area per unit volume (surface density) in all segments reached adult values at approximately 60 days, whilst the increase in absolute area of the surfaces ceased at 90 days in the initial segment and caput and continued to 120 days in the corpus and cauda. The total length of the epididymal tubule showed the same pattern with no increase in length apparent in the initial segment and caput after 90 days. Length however continued to increase in the corpus and cauda between 90 and 120 days. Tubule and luminal diameter reached their definitive values in the initial segment on day 60 but continued to increase until day 90 in the distal segments. Epithelial height increased between 15 and 30 days in all segments; in the caput, epithelial height was greatest on day 30 before decreasing slightly; and in the corpus and cauda maximal epithelial height was observed on day 45 decreasing to stable values on day 60 and 90, respectively. All the data is consistent with a proximal to distal differentiation and development of the epididymis with growth continuing in all segments after the establishment of the definitive architecture. Growth of the distal segments continued after its cessation in the proximal segments. (C) 1994 Wiley-Liss, Inc.