Survival of E-coli O157:H7 in organic wastes destined for land application
Survival of E-coli O157:H7 in organic wastes destined for land application
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DOI：
10.1111/j.1365-2672.2004.02524.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Jones, DL
中科院分区：
文献类型：
--
作者：
Avery, LM;Killham, K;Jones, DL
为确定大肠杆菌O157在对照有机废物扩散到陆地上的持久性，并评估将这些废物处置到陆地上的潜在环境风险。将来自屠宰场、废水处理厂(未处理和处理过的污水)、奶油厂和农场(牛浆)的27个有机废物接种大肠杆菌O157：H7，并在10℃的温度下培养。虽然所有废物中的致病菌数目逐渐下降，尽管同一废物类型的下降速度有所不同，但在测试的有机废物中，77%的有机废物在两个月后仍能存活。长期储存有机废物导致大肠杆菌O157：H7的数量显著和逐渐下降。因此，储存可能是减少用于土地应用的废物的病原体负荷的有用手段。然而，在大多数情况下，长期储存并不能完全消除废物中的O157：H7大肠杆菌。我们的结果表明，目前的立法可能不足以保护环境，防止未经处理的废物将O157：H7污染扩散到陆地。
To determine the persistence of Escherichia coli O157 in contrasting organic wastes spread to land and to assess the potential environmental risk associated with the disposal of these wastes to land.Twenty-seven organic wastes originating from slaughterhouses, wastewater treatment plants (raw and treated sewage), creameries and farms (bovine slurry), were inoculated with E. coli O157:H7 and incubated at 10degreesC. Although pathogen numbers gradually declined in all the wastes, albeit at different rates even in the same waste type, E. coli O157:H7 was still viable in 77% of organic wastes tested after 2 months.Long-term storage of organic wastes led to a significant and gradual decline in E. coli O157:H7 numbers. Consequently, storage may be a useful means of reducing the pathogen load of wastes destined for land application. However, in most cases, long-term storage cannot be expected to completely eliminate E. coli O157:H7 from waste.Our results indicate that current legislation may be insufficient to protect the environment from E. coli O157:H7 contamination from untreated wastes spread to land.