Lab-on-chip device for single cell trapping and analysis
Lab-on-chip device for single cell trapping and analysis
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DOI：
10.1007/s10544-013-9803-7
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Li, Chen-Zhong
中科院分区：
文献类型：
--
作者：
Shah, Pratikkumar;Zhu, Xuena;Li, Chen-Zhong
传统的细胞分析给出的是多个细胞的平均结果，并且假设结果是群体中所有细胞的结果。然而，单细胞研究表明，同一类型的单个细胞可能有很大的差异，这些差异可能在细胞功能中发挥重要作用。这些信息在只研究细胞群体实验方法时可能会被掩盖。为了揭示生物学原理并最终改进疾病的检测和治疗，对单细胞分析的新方法提出了极高的要求。我们建议制作一种芯片上实验室设备来研究高通量单细胞纳米毒性分析。该芯片结合了可独立寻址的有源微井电极，用于细胞操作和分析。我们使用正电泳法快速有效地捕获每个微孔中的单个细胞，并控制单个微孔。我们检测了阻抗特性的变化，以验证微孔中的细胞捕获及其健康状况，并提出了一种新的单细胞测试模型，用于纳米毒性和药物测试。
Traditional cell assay gives us an average result of multiple cells and it is assumed that the resultant is the outcome of all cells in population. However, single cell studies have revealed that individual cells of same type may differ dramatically and these differences may have important role to play in cells functionality. Such information can be obscured in only studying cell population experimental approach. To uncover biological principles and ultimately to improve the detection and treatment of disease, new approaches are highly required to single cell analysis. We propose to fabricate a lab on chip device to study high throughput single cell nanotoxicity analysis. The chip incorporates independently addressable active microwell electrodes for cell manipulation and analysis. We employed positive-dielectrophoresis approach to quickly and efficiently capture single cells in each wells with having control over individual microwells. We examined change in impedance properties to verify cell capture in microwell and its health and present a novel model of single cell assay for nanotoxicity, and drug testing.