Diversity of Babesia spp. in cervid ungulates based on the 18S rDNA and cytochrome c oxidase subunit I phylogenies
Diversity of Babesia spp. in cervid ungulates based on the 18S rDNA and cytochrome c oxidase subunit I phylogenies
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DOI：
10.1016/j.meegid.2019.104060
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发表时间：
2020-01-01
影响因子：
3.2
通讯作者：
Modry, David
中科院分区：
文献类型：
--
作者：
Hrazdilova, Kristyna;Rybarova, Marketa;Modry, David
在欧洲主要以几种鹿为代表的自由放养有蹄类动物是蜱虫的重要宿主和蜱传播感染的宿主。许多研究主要集中在鹿中蜱传病原体的流行，目的是确定它们作为重要人类和牲畜病原体的储存宿主的潜在作用。然而，Babalypp的遗传相似性。形成了一个通常称为进化枝VI的群，该群容纳了鹿的皮氏鹿，使这项任务复杂化，并导致描述了一系列令人困惑的特征不佳的菌株。本研究的目的是解决这个问题，使用两个独立的遗传位点，核18 S rRNA和线粒体细胞色素c氧化酶亚基I基因，用于在平行确定巴氏分离物在自由放养的红色，西卡，和roe鹿在两个地区的共同出现在捷克共和国。与18SrRNA基因相比，COX1基因座在核苷酸水平上表现出明显的种内和种间差异。结果证实了B。divergens，Babalypsp. EU1和B.在研究的鹿物种中发现了capreoli，以及另一种未命名的物种的普遍存在，该物种与以前称为Babelus sp.或Babelus cf.的分类单元相匹配。odocoilei或BabeliCH 1组在整个欧洲的其他几个网站。入侵的西卡鹿进入生活周期的至少三个piroplasmid物种在本地鹿动物群中检测到。B的存在。在牛巴贝斯虫病明显不存在的地区，西卡和红鹿中的分歧者引起了关于寄生虫的流行病学、宿主特异性和分类学地位的重要问题。
Free ranging ungulates, represented in Europe mostly by several deer species, are important hosts for ticks and reservoirs of tick-borne infections. A number of studies have focused on the prevalence of tick borne pathogens in deer chiefly with the aim to determine their potential role as reservoir hosts for important human and livestock pathogens. However, genetic similarity of Babesia spp. forming a group commonly termed as a clade VI that accommodates the deer piroplasms, complicates this task and has led to the description of a bewildering array of poorly characterised strains. This study aims to resolve this issue by using two independent genetic loci, nuclear 18S rRNA and mitochondrial cytochrome c oxidase subunit I genes, used in parallel to identify Babesia isolates in free-ranging red, sika, and roe deer in two areas of their co-occurrence in the Czech Republic. The COX1 loci, in contrast to 18S rRNA gene, shows a clear difference between interspecific and intraspecific variation at the nucleotide level. The findings confirm B. divergens, Babesia sp. EU1 and B. capreoli in studied deer species as well as common presence of another unnamed species that matches a taxon previously referred to as Babesia sp. or Babesia cf. odocoilei or Babesia CH1 group in several other sites throughout Europe. The invasive sika deers enter the life cycle of at least three piroplasmid species detected in native deer fauna. The presence of B. divergens in both sika and red deer in an area where bovine babesiosis is apparently absent raises important questions regarding the epidemiology, host specificity and taxonomic status of the parasite.