One billion years of bZIP transcription factor evolution: Conservation and change in dimerization and DNA-binding site specificity
One billion years of bZIP transcription factor evolution: Conservation and change in dimerization and DNA-binding site specificity
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DOI：
10.1093/molbev/msl211
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发表时间：
2007-03-01
影响因子：
10.7
通讯作者：
Robertson, D. L.
中科院分区：
文献类型：
--
作者：
Amoutzias, G. D.;Veron, A. S.;Robertson, D. L.
基因组时代已经揭示，大量观察到的动物表型依赖于有限数量基因表达模式的变化，这些基因是由大量具有不同特异性的转录因子（tf）介导的。tf的二聚化还可以通过将少量单体结合成具有不同功能的二聚体来增加遗传调控网络流形的复杂性。因此，研究这些二聚化tf的进化对于理解动物进化过程中复杂性如何增加至关重要。我们重点研究了第二大二聚化TFs家族，即碱性亮氨酸拉链（bZIP），并推断了它在动物进化的主要阶段是何时扩展的，以及bZIP dna结合和二聚化功能是如何进化的。具体来说，我们将后生动物的bZIPs分为19个科，并确认了其中至少13个科的古老性质，早于刺胞体的分裂。我们在原口动物-后口动物的最后共同祖先中观察到核心二聚化网络的固定。随后是网络中蛋白质数量的增加，但在脊椎动物出现期间，相互作用伙伴的二聚化没有发生重大变化。总之，bzip是了解TFs在动物进化过程中DNA结合和蛋白质相互作用如何演变的一个很好的模型。
The genomic era has revealed that the large repertoire of observed animal phenotypes is dependent on changes in the expression patterns of a finite number of genes, which are mediated by a plethora of transcription factors (TFs) with distinct specificities. The dimerization of TFs can also increase the complexity of a genetic regulatory network manifold, by combining a small number of monomers into dimers with distinct functions. Therefore, studying the evolution of these dimerizing TFs is vital for understanding how complexity increased during animal evolution. We focus on the second largest family of dimerizing TFs, the basic-region leucine zipper (bZIP), and infer when it expanded and how bZIP DNA-binding and dimerization functions evolved during the major phases of animal evolution. Specifically, we classify the metazoan bZIPs into 19 families and confirm the ancient nature of at least 13 of these families, predating the split of the cnidaria. We observe fixation of a core dimerization network in the last common ancestor of protostomes-deuterostomes. This was followed by an expansion of the number of proteins in the network, but no major dimerization changes in interaction partners, during the emergence of vertebrates. In conclusion, the bZIPs are an excellent model with which to understand how DNA binding and protein interactions of TFs evolved during animal evolution.