QUANTITATIVE HISTOCHEMICAL DETERMINATION OF MUSCLE ENZYMES - BIOCHEMICAL VERIFICATION
QUANTITATIVE HISTOCHEMICAL DETERMINATION OF MUSCLE ENZYMES - BIOCHEMICAL VERIFICATION
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DOI：
10.1177/33.10.4045183
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
CASTLEMAN, KR
中科院分区：
文献类型：
--
作者：
MARTIN, TP;VAILAS, AC;CASTLEMAN, KR
我们建立了琥珀酸脱氢酶和α-葡萄糖苷酶的酶活性的定量组织化学测定。猫骨骼肌甘油磷酸脱氢酶。使用计算机增强图像分析系统对组织化学酶活性反应产物进行定量。我们描述了一系列的实验，验证的可靠性和有效性的测定。组织化学测定的酶活性与组织厚度和反应时间呈线性关系。 生化测定的酶活性也与组织厚度和孵育时间呈线性关系。使用组织化学或生物化学测定的连续组织切片来建立线性回归方程，该线性回归方程允许定量组织化学测定的反应速率（以每分钟光密度测量）被校准为纳摩尔/分钟。
We established quantitative histochemical assays for the enzymatic activity of succinate dehydrogenase and .alpha.-glycerol phosphate dehydrogenase for cat skeletal muscle. A computer-enhanced image analysis system was used to quantitate the histochemical enzyme-activity reaction products. We describe a series of experiments that verify the reliability and validity of the assays. Histochemically determined enzyme activities were linear with respect to tissue thickness and reaction time. Biochemically determined enzyme activities were also linear with respect to tissue thickness and incubation time. Consecutive tissue sections, assayed either histochemically or biochemically, were used to establish a linear regression equation that allowed quantitative histochemically determined reaction rates, measured in optical density per minute, to be calibrated as nanomoles per minute.