Heparanase expression and TrkC/p75NTR ratios in human medulloblastoma.
Heparanase expression and TrkC/p75NTR ratios in human medulloblastoma.
复制标题
人髓母细胞瘤中乙酰肝素酶的表达和 TrkC/p75NTR 比率。
DOI：
10.1007/s10585-006-9017-y
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发表时间：
2006
影响因子：
4
通讯作者：
Marchetti,Dario
中科院分区：
文献类型：
--
作者：
Sinnappah-Kang,NeetaD;Mrak,RobertE;Paulsen,DanielB;Marchetti,Dario
Medulloblastoma (MB), the most devastating and common brain tumor in children, is highly invasive and extremely difficult to treat. Identifying the properties of MB tumors that cause them to invade and metastasize is therefore imperative for the development of novel treatments. We performed investigations to elucidate prognostic implications of heparanase (HPSE-1) and TrkC/p75NTRexpression in MB using formalin-fixed, paraffin-embedded (FFPE) MB clinical specimens from children aged 1–19 years. Expressions of p75NTRand HPSE-1 correlated with each other (Pearson’s correlationR= 0.899;P< 0.0001;R2= 81%;n= 14). In addition, TrkC:p75NTRratios correlated with MB meningeal spread (R= 0.608;P= 0.0212;R2= 37%;n= 14). Secondly, using antibodies specific to TrkC and HPSE-1, we carried out immunohistochemistry (IHC) on 22 human MB tissue samples. IHC reaction scores revealed a significant expression of HPSE-1 in 76% of MB tissues from children aged 3 years and older (P= 0.0490;n= 17) while TrkC immunoreactivity was detected in 71% of these patient samples. Of note, TrkC was significantly present in 100% of MB female patients (P= 0.0313;n= 6). These studies support the role of p75NTRand HPSE-1 as two novel molecular determinants involved in the biology and clinical progression of MB.
