Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability.
Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability.
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DOI：
10.1016/j.actbio.2016.03.022
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发表时间：
2016-05
影响因子：
9.7
通讯作者：
Leipzig ND
中科院分区：
文献类型：
--
作者：
Patil PS;Fountas-Davis N;Huang H;Michelle Evancho-Chapman M;Fulton JA;Shriver LP;Leipzig ND
本研究以全氟碳链修饰的甲基丙烯酰胺壳聚糖(MACF)为基料，制备皮肤创面水凝胶敷料。在动物模型中，测试了饱和氧气(+O2)的MACF水凝胶输送和维持氧气、在生物环境中降解以及促进伤口愈合的能力。这项新兴的代谢组学技术被用来了解MACF+O2水凝胶敷料如何促进伤口愈合。结果表明，MACF处理促进了氧传输速率，比基础MAC水凝胶大两个数量级。MACF水凝胶敷料接下来在活体夹板大鼠切除伤口愈合模型中进行测试。组织学分析显示，MACF+O2敷料可促进再上皮化(p<；0.0001)和胶原合成(p<；0.01)。利用全球代谢组学对创面内源性代谢物的分析表明，MACF+O2敷料促进了指向羟脯氨酸和胶原合成的再生代谢过程，并证实了这一途径中的代谢物水平。这项研究的结果证实，通过应用MACF+O2水凝胶而增加的氧气输送促进了伤口的愈合，代谢组学分析为评估伤口愈合生理提供了强有力的工具。
In this study, methacrylamide chitosan modified with perfluorocarbon chains (MACF) is used as the base material to construct hydrogel dressings for treating dermal wounds. MACF hydrogels saturated with oxygen (+ O2) are examined for their ability to deliver and sustain oxygen, degrade in a biological environment, and promote wound healing in an animal model. The emerging technique of metabolomics is used to understand how MACF + O2 hydrogel dressings improve wound healing. Results indicate that MACF treatment facilitates oxygen transport rate that is two orders of magnitude greater than base MAC hydrogels. MACF hydrogel dressings are next tested in an in vivo splinted rat excisional wound healing model. Histological analysis reveals that MACF + O2 dressings improve re-epithelialization (p < 0.0001) and synthesis of collagen over controls (p < 0.01). Analysis of endogenous metabolites in the wounds using global metabolomics demonstrates that MACF + O2 dressings promotes a regenerative metabolic process directed toward hydroxyproline and collagen synthesis, with confirmation of metabolite levels within this pathway. The results of this study confirm that increased oxygen delivery through the application of MACF + O2 hydrogels enhances wound healing and metabolomics analyses provides a powerful tool to assess wound healing physiology.