Association of EphA4 polymorphism with swine reproductive traits and mRNA expression of EphA4 during embryo implantation
Association of EphA4 polymorphism with swine reproductive traits and mRNA expression of EphA4 during embryo implantation
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DOI：
10.1007/s11033-011-1023-8
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Wang, Aiguo
中科院分区：
文献类型：
--
作者：
Fu, Yanfeng;Fu, Jinluan;Wang, Aiguo
猪促红细胞生成素肝细胞A4(EphA4)基因在猪胚胎着床过程中发现于子宫内膜，是影响繁殖性状的潜在候选基因之一。本研究对EphA4基因外显子3的两个单核苷酸多态(SNP)基因座(EphA4_1和EphA4_2)进行了分析，以确定EphA4对新生儿总数(TNB)和活产仔数(NBA)的影响。对长白猪、约克夏母猪和杜洛克母猪的2,014窝母猪繁殖性状与两个双等位基因多态的相关性进行了研究。结果表明，EphA4_1座位上的G等位基因或EphA4_2座位上的C等位基因似乎对产仔数有有利影响。综合分析结果表明，无论是单胎还是所有胎次，AGTC、AGCC和GGCC基因型均优于AATT、AATC和AGTT基因型。本研究结果表明，EphA4基因与猪产仔数显著相关。此外，EphA4在猪的小肠、大肠、胃和子宫内膜中也有较高的表达，但在着床过程中表达下降。需要进一步的研究来证实这些初步研究。
The swine erythropoietin-producing hepatocellular A4 (EphA4) gene, which was detected in the endometrium during embryo implantation in pigs, was one of the potential candidate genes for reproductive traits. In the study, two single nucleotide polymorphism (SNP) loci (EphA4_1 and EphA4_2) in exon 3 of EphA4 gene were analyzed to determine whether EphA4 influenced total number born (TNB) and number born alive (NBA). Association of two diallelic polymorphisms with reproductive traits was assessed in Landrace, Yorkshire and Duroc populations with 2,014 litter records of 765 sows. The results showed that G allele at EphA4_1 locus or C allele at EphA4_2 locus seemed to have advantageous effects on litter size. And the combined analyzed results demonstrated that genotype AGTC, AGCC and GGCC are better than genotype AATT, AATC and AGTT for TNB and NBA in either single parity or all parities. The results in this study demonstrated that EphA4 gene was significantly associated with litter size in pigs. In addition, a high mRNA expression of EphA4 was found in small intestine, large intestine, stomach and endometrium, and the expression decreased during implantation in pigs. Further studies were needed to confirm these preliminary researches.