Chemotaxis assays of mouse sperm on microfluidic devices
Chemotaxis assays of mouse sperm on microfluidic devices
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DOI：
10.1021/ac052087i
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发表时间：
2006-05-15
影响因子：
7.4
通讯作者：
Jacobson, Stephen C.
中科院分区：
文献类型：
--
作者：
Koyama, Sachiko;Amarie, Dragos;Jacobson, Stephen C.
精子趋化性是生殖科学科学家非常感兴趣的一个领域。了解精子细胞是如何以及何时被卵子吸引的，可能会对生殖和避孕产生深远的影响。为了系统地研究这一问题，我们制作并评估了一种测量精子趋化性的微流控装置。该装置的设计采用了空间和时间稳定的化学梯度的流过结构。将小鼠精子细胞引入小鼠卵巢提取液和缓冲液的汇合流之间的趋化室。经历趋化性的精子游向提取液，并相对于游向缓冲液的精子进行计数。卵巢提取物的稀释倍数为10(2)~10(7)倍，并对每次稀释的提取物进行趋化筛选。6个卵巢中有4个在提取物稀释度为10(-3)~10(-5)时表现出强烈的趋化反应。这种设备提供了一个方便的、一次性的平台，在上面进行趋化性分析，流通式设计克服了与区分趋化性和捕获相关的困难。
Sperm chemotaxis is an area of significant interest to scientists involved in reproductive science. Understanding how and when sperm cells are attracted to the egg could have profound effects on reproduction and contraception. In an effort to systematically study this problem, we have fabricated and evaluated a microfluidic device to measure sperm chemotaxis. The device was designed with a flowthrough configuration using a spatially and temporally stable chemical gradient. Mouse sperm cells were introduced into the chemotaxis chamber between confluent flows of mouse ovary extract and buffer. The sperm experiencing chemotaxis swam toward the extract and were counted relative to those that swam toward the buffer. The ovary extracts were diluted from 10(2) to 10(7) times, and each extract dilution was screened for chemotaxis. Four out of six ovaries showed a strong chemotactic response at extract dilutions of 10(-3) to 10(-5). This device provided a convenient, disposable platform on which to conduct chemotaxis assays, and the flow-through design overcomes difficulties associated with distinguishing chemotaxis from trapping.