Proteome modifications of gut microbiota in mice with activity-based anorexia and starvation: Role in ATP production
Proteome modifications of gut microbiota in mice with activity-based anorexia and starvation: Role in ATP production
复制标题
DOI：
10.1016/j.nut.2019.110557
复制
发表时间：
2019-11-01
期刊：
影响因子：
4.4
通讯作者：
Fetissov, Serguei O.
中科院分区：
文献类型：
--
作者：
Breton, Jonathan;Legrand, Romain;Fetissov, Serguei O.
目的：活动性厌食症（Activity-based anorexia， ABA）是啮齿动物神经性厌食症的行为模式，其特征为负能量平衡、多动、肠道菌群失调。众所周知，肠道细菌可以产生能量底物，包括三磷酸腺苷（ATP）和醋酸酯。本研究的目的是确定ABA是否会改变与ATP和醋酸盐生产相关的肠道微生物群的蛋白质组。方法：在雄性小鼠体内制备ABA，并与限食和自由喂养条件下进行比较。采用二维凝胶电泳和质谱法对粪便进行蛋白质组学分析。测定从粪便中提取的蛋白质体外产生atp的能力。结果：限食小鼠粪便中磷酸甘油酸激酶（一种产生atp的糖酵解酶）水平升高，ABA组该酶水平进一步升高。饥饿还上调了梭状芽孢杆菌目（包括梭状芽孢杆菌科和Lachnospiracetic科）合成的其他几种蛋白质。来自ABA、限食和自由喂养的对照组小鼠的细菌蛋白在体外产生atp的能力没有显著差异。然而，在包括ABA小鼠在内的活性组中，血浆乙酸水平有明显增加的趋势。结论：限食小鼠和ABA小鼠的饥饿诱导肠道细菌的蛋白质组改变，主要是梭状芽胞杆菌产生ATP。然而，这并没有导致肠道微生物群总atp生产能力的增加。这些变化可以解释为特定肠道细菌对宿主营养不良的适应，有利于宿主的生存。(C) 2019 Elsevier Inc.版权所有。
Objective: Activity-based anorexia (ABA) in rodents is a behavioral model of anorexia nervosa, characterized by negative energy balance, hyperactivity, and dysbiosis of gut microbiota. Gut bacteria are known to produce energy substrates including adenosine triphosphate (ATP) and acetate. The aim of this study was to determine whether ABA alters the proteome of gut microbiota relevant to ATP and acetate production.Methods: The ABA was developed in male mice and compared with food-restricted and ad libitum-fed conditions. Proteomic analysis of feces was performed using the two-dimentional gel electrophoresis and mass spectrometry. The in vitro ATP-producing capacity of proteins extracted from feces was assayed.Results: Increased levels of the phosphoglycerate kinase, an ATP-producing glycolytic enzyme, was detected in feces of food-restricted mice and this enzyme was further increased in the ABA group. Starvation also upregulated several other proteins synthetized by order Clostridiales including Clostridiaceae and Lachnospiracetic families. No significant differences in the in vitro ATP-producing capacity by bacterial proteins from ABA, food-restricted, and ad libitum-fed control mice were found. However, plasma levels of acetate strongly tended to be increased in the activity groups including ABA mice.Conclusion: The data revealed that starvation in food-restricted and ABA mice induced proteome modification in gut bacteria favoring ATP production mainly by the order Clostridiales. However, this did not result in increased total ATP-production capacity by gut microbiota. These changes can be interpreted as an adaptation of specific gut bacteria to the host malnutrition beneficial for host survival. (C) 2019 Elsevier Inc. All rights reserved.