Analysis of the genome sequence of the pathogenic Muscovy duck parvovirus strain YY reveals a 14-nucleotide-pair deletion in the inverted terminal repeats
Analysis of the genome sequence of the pathogenic Muscovy duck parvovirus strain YY reveals a 14-nucleotide-pair deletion in the inverted terminal repeats
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对致病性番鸭细小病毒株 YY 的基因组序列分析显示，反向末端重复序列中有 14 个核苷酸对缺失。
DOI：
10.1007/s00705-016-2946-0
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发表时间：
2016-09-01
影响因子：
2.7
通讯作者：
Zhu, Guoqiang
中科院分区：
文献类型：
--
作者：
Wang, Jianye;Huang, Yu;Zhu, Guoqiang
关于番鸭细小病毒的基因组信息仍然有限。本研究对MDPV致病株YY株的基因组进行了测序。YY株基因组全长5075个核苷酸，比FM株短57个核苷酸。序列比对表明，与菌株FM和FZ91-30相比，YY株的5‘和3’反向末端重复序列(ITR)在回文发夹结构的茎中含有14个核苷酸对的缺失。缺失的区域包含一个“E-box”位点和一个重复基序“TTCCGGT”或“ACCGGAA”。根据蛋白质编码基因构建的系统发育树表明，YY与其他9个来自不同地区的MDPV分离株聚在一个独立的分支上，与鹅细小病毒(GPV)毒株形成的分支不同。这些结果表明，尽管YY株存在明显的缺失，但仍属于典型的MDPV组。此外，ITR基因的缺失可能参与了免疫压力下MDPV的基因组进化。
Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR) of strain YY contain a 14-nucleotide-pair deletion in the stem of the palindromic hairpin structure in comparison to strain FM and FZ91-30. The deleted region contains one "E-box" site and one repeated motif with the sequence "TTCCGGT" or "ACCGGAA". Phylogenetic trees constructed based the protein coding genes concordantly showed that YY, together with nine other MDPV isolates from various places, clustered in a separate branch, distinct from the branch formed by goose parvovirus (GPV) strains. These results demonstrate that, despite the distinctive deletion, the YY strain still belongs to the classical MDPV group. Moreover, the deletion of ITR may contribute to the genome evolution of MDPV under immunization pressure.