Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi
Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi
复制标题
DOI：
10.1128/jb.186.20.6902-6914.2004
复制
发表时间：
2004-10-01
影响因子：
3.2
通讯作者：
Bassler, BL
中科院分区：
文献类型：
--
作者：
Henke, JM;Bassler, BL
在一个称为群体感应的过程中，细菌使用称为自诱导物的细胞外信号分子进行交流。两个平行的群体感应系统已被确定在海洋细菌哈氏弧菌。系统1由LuxM依赖性自诱导剂HAI-1和HAI-1传感器LuxN组成。系统2由LuxS依赖性自诱导剂AI-2和AI-2检测剂LuxPQ组成。相关的细菌，霍乱弧菌，一种人类病原体，拥有系统2（LuxS，AI-2和LuxPQ），但没有明显的哈维氏弧菌系统1的同源物。相反，霍乱弧菌的系统1由CqsA依赖性自诱导物CAI-1和称为CqsS的传感器组成。使用霍乱弧菌CAI-1报告菌株，我们表明许多其他海洋细菌，包括哈维氏弧菌，产生CAI-1活性。哈维氏弧菌的遗传分析揭示了cqsA和cqsS，哈维氏弧菌cqsA和cqsS突变体的表型分析表明，这些功能构成了与系统1和2平行作用的第三个哈维氏弧菌群体感应系统。这些通讯系统共同作为一个三向符合检测器在各种基因的调节，包括那些负责生物发光，III型分泌，和金属蛋白酶的生产。
In a process called quorum sensing, bacteria communicate using extracellular signal molecules termed autoinducers. Two parallel quorum-sensing systems have been identified in the marine bacterium Vibrio harveyi. System 1 consists of the LuxM-dependent autoinducer HAI-1 and the HAI-1 sensor, LuxN. System 2 consists of the LuxS-dependent autoinducer AI-2 and the AI-2 detector, LuxPQ. The related bacterium, Vibrio cholerae, a human pathogen, possesses System 2 (LuxS, AI-2, and LuxPQ) but does not have obvious homologues of V harveyi System 1. Rather, System 1 of V. cholerae is made up of the CqsA-dependent autoinducer CAI-1 and a sensor called CqsS. Using a V. cholerae CAI-1 reporter strain we show that many other marine bacteria, including V. harveyi, produce CAI-1 activity. Genetic analysis of V. harveyi reveals cqsA and cqsS, and phenotypic analysis of V. harveyi cqsA and cqsS mutants shows that these functions comprise a third V. harveyi quorum-sensing system that acts in parallel to Systems 1 and 2. Together these communication systems act as a three-way coincidence detector in the regulation of a variety of genes, including those responsible for bioluminescence, type III secretion, and metalloprotease production.