INTEGRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA INVITRO
INTEGRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA INVITRO
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DOI：
10.1073/pnas.87.11.4164
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发表时间：
1990-06-01
影响因子：
11.1
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
--
作者：
FARNET, CM;HASELTINE, WA
描述了一种用于整合人类免疫缺陷病毒1型DNA的高效无细胞系统。线状病毒DNA合成出现在新感染细胞的细胞质中，在感染后4小时达到峰值。存在于细胞质提取液中的线性病毒DNA分子能够在体外整合到异源DNA靶标中。病毒DNA存在于高分子量核蛋白结构中，这种结构可以从大部分细胞蛋白质和核酸中分离出来，而不会明显降低体外整合的能力。
A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.