Exercise training improves cardiac function in infarcted rabbits: involvement of autophagic function and fatty acid utilization
Exercise training improves cardiac function in infarcted rabbits: involvement of autophagic function and fatty acid utilization
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DOI：
10.1093/eurjhf/hfq028
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发表时间：
2010-04-01
影响因子：
18.2
通讯作者：
Chen, Ming-Fong
中科院分区：
文献类型：
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作者：
Chen, Ching-Yi;Hsu, Hsiu-Ching;Chen, Ming-Fong
目的探讨运动能否改善心肌梗死(MI)后兔的心功能，探讨运动对左心室功能的影响。方法成年雄性新西兰大白兔(体重2.5~3 kg)，结扎左冠状动脉前降支造成心肌梗死。术后8周，假手术组和心肌梗死后组动物在静坐状态下或每周5天以1.0公里/小时的速度进行为期4周的30min跑台运动，然后处死动物。取左室非梗死区作进一步分析。MI使左心室射血分数(LVEF)降低，硫代巴比妥酸反应物质(TBARS)生成增加。运动可改善心肌梗死兔的心功能。心肌梗死组左心室微管相关蛋白轻链3(MAPL3)的LC3II/LC3I较假手术组高2.1倍，运动组LC3II/LC3I明显降低。MI可下调心肌型脂肪酸结合蛋白(h-FABP)的表达，运动可上调h-FABP的表达。LVEF与h-FABP呈显著正相关，与LC_(33)/LC_(33)呈显著负相关。结论运动引起的自噬功能改变和脂肪酸利用可能有助于心肌梗死后心功能的改善。
Aims To explore whether exercise can improve cardiac function in a post-myocardial infarction (MI) rabbit model and to determine contributing factors in the left ventricle (LV).Methods and results Adult male New Zealand White rabbits (2.5-3 kg) underwent MI by ligation of the left anterior descending coronary artery. For 8 weeks after surgery, sham-operated, and post-MI rabbits were housed under sedentary conditions or assigned to a 4-week treadmill exercise protocol at a speed of 1.0 km/h for 30 min 5 days per week, then sacrificed. The non-infarcted region of the LV was harvested for further analysis. MI decreased left ventricular ejection fraction (LVEF) and increased thiobarbituric acid reactive substances (TBARS) generation in the LV. Exercise improved the cardiac function of MI rabbits. Left ventricular LC3II/LC3I (microtubule-associated protein light chain 3) in the MI group was 2.1-fold higher than that of the sham group, exercise significantly decreased LC3II/LC3I in the MI group. MI down-regulated the expression of heart-type fatty acid binding protein (h-FABP), and exercise up-regulated h-FABP. In addition, LVEF had a significantly positive correlation with h-FABP and a negative correlation with LC3II/LC3I.Conclusion Exercise induced change in autophagic function and fatty acid utilization may contribute to the improvement in ventricular function in the infarcted heart.