Protein Vesicles Self-Assembled from Functional Globular Proteins with Different Charge and Size
Protein Vesicles Self-Assembled from Functional Globular Proteins with Different Charge and Size
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DOI：
10.1021/acs.biomac.0c00671
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发表时间：
2021-01-01
影响因子：
6.2
通讯作者：
Champion, Julie A.
中科院分区：
文献类型：
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作者：
Dautel, Dylan R.;Champion, Julie A.
蛋白质囊泡可以通过混合两种融合蛋白来合成：与富含谷氨酸的亮氨酸拉链（Z（R））融合的弹性蛋白样多肽（ELP）和与富含谷氨酸的亮氨酸拉链（Z（E））融合的球状可溶性蛋白。目前，只有荧光蛋白已被纳入囊泡;然而，蛋白质vesides是有用的生物催化，药物输送，或生物传感，vesides必须组装从功能蛋白质，跨越一系列的属性和功能。在这项工作中，系统地改变了球状蛋白质，以确定表面电荷和大小对蛋白质血管自组装的影响。微相的形成，其中包括囊泡，凝聚体，和混合结构，在不同的组装条件下进行监测，以确定每个球状蛋白的相空间。结果表明，蛋白质表面电荷对囊泡自组装的影响不大。然而，增加球状蛋白的尺寸减小囊泡尺寸，并在较低的Z（E）/Z（R）摩尔比下增加稳定性。所创建的相图可以用作将新的功能蛋白质并入囊泡的指南。此外，这项工作报告催化活性的酶vesides，展示了潜在的应用囊泡作为生物催化剂或生物传感器。
Protein vesicles can be synthesized by mixing two fusion proteins: an elastin-like polypeptide (ELP) fused to an argininerich leucine zipper (Z(R)) with a globular, soluble protein fused to a glutamate-rich leucine zipper (Z(E)). Currently, only fluorescent proteins have been incorporated into vesicles; however, for protein vesides to be useful for biocatalysis, drug delivery, or biosensing, vesides must assemble from functional proteins that span an array of properties and functionalities. In this work, the globular protein was systematically changed to determine the effects of the surface charge and size on the self-assembly of protein vesides. The formation of microphases, which included vesicles, coacervates, and hybrid structures, was monitored at different assembly conditions to determine the phase space for each globular protein. The results show that the protein surface charge has a small effect on vesicle self-assembly. However, increasing the size of the globular protein decreases the vesicle size and increases the stability at lower Z(E)/Z(R) molar ratios. The phase diagrams created can be used as guidelines to incorporate new functional proteins into vesicles. Furthermore, this work reports catalytically active enzyme vesides, demonstrating the potential for the application of vesicles as biocatalysts or biosensors.