Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.
Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.
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姜黄素通过抑制 Wnt/β-catenin 和 Sonic Hedgehog 通路来抑制肺癌干细胞。
DOI：
10.1002/ptr.5791
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hong-Yu Han
中科院分区：
文献类型：
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作者：
Jian-Yun Zhu;Xue Yang;Yue Chen;Ye Jiang;Shi-Jia Wang;Yuan Li;Xiao-Qian Wang;Yu Meng;Ming-Ming Zhu;Xiao Ma;Cong Huang;Rui Wu;Chun-Feng Xie;Xiao-Ting Li;Shan-Shan Geng;Jie-Shu Wu;Cai-Yun Zhong;Hong-Yu Han
Cancer stem cells (CSCs) are highly implicated in the progression of human cancers. Thus, targeting CSCs may be a promising strategy for cancer therapy. Wnt/β‐catenin and Sonic Hedgehog pathways play an important regulatory role in maintaining CSC characteristics. Natural compounds, such as curcumin, possess chemopreventive properties. However, the interventional effect of curcumin on lung CSCs has not been clarified. In the present study, tumorsphere formation assay was used to enrich lung CSCs from A549 and H1299 cells. We showed that the levels of lung CSC markers (CD133, CD44, ALDHA1, Nanog and Oct4) and the number of CD133‐positive cells were significantly elevated in the sphere‐forming cells. We further illustrated that curcumin efficiently abolished lung CSC traits, as evidenced by reduced tumorsphere formation, reduced number of CD133‐positive cells, decreased expression levels of lung CSC markers, as well as proliferation inhibition and apoptosis induction. Moreover, we demonstrated that curcumin suppressed the activation of both Wnt/β‐catenin and Sonic Hedgehog pathways. Taken together, our data suggested that curcumin exhibited its interventional effect on lung CSCs via inhibition of Wnt/β‐catenin and Sonic Hedgehog pathways. These novel findings could provide new insights into the potential therapeutic application of curcumin in lung CSC elimination and cancer intervention. Copyright © 2017 John Wiley & Sons, Ltd.
