MYB76 Inhibits Seed Fatty Acid Accumulation in Arabidopsis
MYB76 Inhibits Seed Fatty Acid Accumulation in Arabidopsis
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MYB76 抑制拟南芥种子脂肪酸积累
DOI：
10.3389/fpls.2017.00226
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发表时间：
2017-02-21
影响因子：
5.6
通讯作者：
Chen, Mingxun
中科院分区：
文献类型：
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作者：
Duan, Shaowei;Jin, Changyu;Chen, Mingxun
MYB家族转录因子在拟南芥的发育、代谢以及对生物和非生物胁迫的响应等调控网络中起着重要作用。然而，它们在调节种子中脂肪酸积累中的作用在很大程度上仍然不清楚。在这里，我们发现MYB 76，定位于细胞核，主要是在成熟过程中发育的种子中表达。MYB76突变导致成熟种子中总脂肪酸和几种主要脂肪酸组成的量显著增加，表明MYB76在种子油生物合成过程中起着重要的阻遏物的作用。RNA测序和实时荧光定量PCR分析显示，在授粉后12天的myb76种子的光合作用，脂肪酸的生物合成，修饰和降解，和油体形成的许多基因的显着改变。这些结果有助于我们理解MYB 76的新功能，并为MYB转录因子控制拟南芥种子油积累的调控网络提供新的见解。
The MYB family of transcription factors is important in regulatory networks controlling development, metabolism and responses to biotic and abiotic stresses in Arabidopsis. However, their role in regulating fatty acid accumulation in seeds is still largely unclear. Here, we found that MYB76, localized in the nucleus, was predominantly expressed in developing seeds during maturation. The myb76 mutation caused a significant increase in the amounts of total fatty acids and several major fatty acid compositions in mature seeds, suggesting that MYB76 functioned as an important repressor during seed oil biosynthesis. RNA sequencing and quantitative real-time PCR analysis revealed remarkable alteration of numerous genes involved in photosynthesis, fatty acid biosynthesis, modification, and degradation, and oil body formation in myb76 seeds at 12 days after pollination. These results help us to understand the novel function of MYB76 and provide new insights into the regulatory network of MYB transcriptional factors controlling seed oil accumulation in Arabidopsis.