Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage
Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage
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DOI：
10.1080/10715760500161165
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发表时间：
2005-08-01
影响因子：
3.3
通讯作者：
Hyun, JW
中科院分区：
文献类型：
--
作者：
Kang, KA;Lee, KH;Hyun, JW
本研究从海兰中分离得到一种根皮鞣质，并对其抗氧化性能进行了研究。三间苯二甲醇-A能抑制细胞内活性氧自由基（ROS）和DPPH自由基，从而防止脂质过氧化。雷公藤多甙A清除自由基的活性可通过激活ERK蛋白而保护过氧化氢（H2 O2）诱导的中国仓鼠肺成纤维细胞（V79-4）免于细胞死亡。此外，曲叶菊醇-A减少H2 O2诱导的凋亡细胞的形成。雷公藤多甙A能提高细胞内抗氧化酶的活性，如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）。因此，从本研究中，这是提示，曲苯二甲醇-A保护V79-4细胞免受H2 O2损伤，通过增强细胞的抗氧化活性。
In the present study, triphlorethol-A, a phlorotannin, was isolated from Ecklonia cava and its antioxidant properties were investigated. Triphlorethol-A was found to scavenge intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl ( DPPH) radical, and thus prevented lipid peroxidation. The radical scavenging activity of triphlorethol-A protected the Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H2O2) against cell death, via the activation of ERK protein. Furthermore, triphlorethol-A reduced the apoptotic cells formation induced by H2O2. Triphlorethol-A increased the activities of cellular antioxidant enzymes like, superoxide dismutase ( SOD), catalase ( CAT) and glutathione peroxidase (GPx). Hence, from the present study, it is suggestive that triphlorethol-A protects V79-4 cells against H2O2 damage by enhancing the cellular antioxidative activity.