Genotypic differences in cadmium uptake and distribution in soybeans
Genotypic differences in cadmium uptake and distribution in soybeans
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DOI：
10.1023/a:1023079819086
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发表时间：
2003-04-01
期刊：
影响因子：
4.9
通讯作者：
Takahashi, M
中科院分区：
文献类型：
--
作者：
Arao, T;Ae, N;Takahashi, M
为了研究大豆对镉的吸收和分配的遗传差异，将17个大豆品种先在土壤中种植，然后将4 ~ 5个大豆品种在不同镉水平的营养液中种植。田间和盆栽试验结果表明，不同基因型大豆籽粒镉含量存在显著差异，En-b 0 -1-2籽粒镉含量最低，Harosoy籽粒镉含量最高。东北128的种子镉含量介于双亲之间。对于四种土壤类型，从0.2到6.5毫克kg（-1）的可提取镉，大豆基因型的排名种子镉水平的基础上是相似的，表明有一个遗传因素参与镉浓度的品种差异。在本研究的一个试验中，在四个大豆品种中，叶，茎和豆荚中的镉浓度以及总镉吸收量最低的En-b 0 -1-2。这些结果表明，镉的吸收和/或转运从根到地上部是低的En-b 0 -1-2。在含有100 μ g L-1镉的溶液培养中，在种子成熟阶段的种子，茎和豆荚中的镉浓度也是最低的En-b 0 -1-2。在第二个实验中，镉的叶，茎和叶柄中的镉浓度在7和15天后，En-b 0 -1-2和Enrei低于东北128，铃丰和Harosoy的营养液中添加镉，但根中的镉浓度En-b 0 -1-2和Enrei高于其他品种。我们建议，在某些品种的大豆种子中发现的较低水平的镉的结果从较低的初始吸收和较高水平的镉在根中的保留相结合，从而限制其转运到地上部。
In order to investigate the genetic differences in uptake and distribution of cadmium in soybeans, 17 varieties of soybean were grown first in soil and then four or five varieties of soybean were grown in nutrient solution with different levels of cadmium. Significant genotypic differences in seed cadmium levels were found. The seed cadmium concentration was lowest for the En-b0-1-2 soybean variety, and highest for Harosoy, in both field and pot experiments. The seed cadmium levels of Tohoku 128, a cross between Enrei and Suzuyutaka, were intermediate between those of the parents. For four soil types, containing from 0.2 to 6.5 mg kg(-1) extractable cadmium, the ranking of soybean genotypes based on seed cadmium level was similar, indicating that there is a genetic factor involved in the varietal differences in cadmium concentration. Among the four soybean varieties tested in one experiment in the present study, the cadmium concentrations in leaves, stems and pods as well as the total cadmium uptake were lowest for En-b0-1-2. These results suggest that cadmium uptake and/or translocation from root to shoot are low in En-b0-1-2. In solution culture containing 100 mug L-1 cadmium, the cadmium concentrations in seeds, stems and pods at the seed maturation stage were also the lowest for En-b0-1-2. In a second experiment, the cadmium concentrations in the leaves, stem and petiole were lower at both 7 and 15 days after the addition of cadmium to the nutrient solution for En-b0-1-2 and Enrei than for Tohoku 128, Suzuyutaka and Harosoy; however, the cadmium concentrations of roots for En-b0-1-2 and Enrei were higher than for the other varieties. We propose that the lower levels of cadmium found in the seeds of certain varieties of soybean result from the combination of lower initial uptake and retention of higher levels of cadmium in the roots, thus limiting its translocation to the shoot.