Dibutyryl cyclic AMP induces formyl peptide receptor expression and chemotactic responses in a human eosinophilic cell line, EoL-1.
Dibutyryl cyclic AMP induces formyl peptide receptor expression and chemotactic responses in a human eosinophilic cell line, EoL-1.
复制标题
二丁酰环 AMP 在人嗜酸性细胞系 EoL-1 中诱导甲酰肽受体表达和趋化反应。
作者：
Y. Uenoyama;Y. Ohshima;M. Morita;H. Akutagawa;M. Nambu;K. M. Kim;M. Mayumi;H. Mikawa
我们研究了肌动蛋白聚合和细胞内游离钙离子浓度（[Ca 2 +]i）的增加，在人嗜酸性粒细胞白血病细胞系，EoL-1，在刺激与趋化因子，我们还研究了双丁酰环腺苷酸（dbcAMP）的反应的影响。EoL-1细胞在正常培养条件下没有表现出任何肌动蛋白聚合或[Ca 2 +]i增加时，刺激与甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）。在未处理的EoL-1细胞上也检测不到甲酰肽受体的表达。双丁酰cAMP诱导甲酰基肽受体的表达和对fMLP的反应性。dbcAMP还诱导或增强EoL-1细胞对补体片段C5 a和血小板活化因子（PAF）的反应性。dbcAMP处理EoL-1细胞后，EoL-1细胞生长速度减慢，G 0/G1期细胞比例增加。dbcAMP刺激EoL-1细胞较长时间后，EoL-1细胞内嗜酸性粒细胞比例和嗜酸性粒细胞阳离子蛋白（ECP）含量增加。EoL-1细胞对fMLP、C5 a和PAF的反应性显示出独立调节。EoL-1细胞和dbcAMP似乎是有用的检查趋化受体的表达和其信号转导机制。
We investigated actin polymerization and the increase of cytosolic free calcium concentration ([Ca2+]i) in a human eosinophilic leukemia cell line, EoL-1, in response to stimulation with chemotactic factors; we also investigated the effect of dibutyryl cyclic AMP (dbcAMP) on the responses. EoL-1 cells under normal culture conditions did not show either actin polymerization or an increase in [Ca2+]i when stimulated with formyl-methionyl-leucyl-phenylalanine (fMLP). Expression of formyl peptide receptors was not detectable on untreated EoL-1 cells, either. Dibutyryl cAMP induced the expression of formyl peptide receptors and the responsiveness to fMLP. The responsiveness of EoL-1 cells to the complement fragment C5a and platelet-activating factor (PAF) was also induced or enhanced by dbcAMP. The growth of EoL-1 cells was decreased and the proportion of cells in the G0/G1 phase of the cell cycle was increased by the treatment of EoL-1 cells with dbcAMP. The proportion of eosinophilic granule-containing cells and the content of eosinophil cationic protein (ECP) in EoL-1 cells was also increased when they were stimulated with dbcAMP for a longer period. The responsiveness of EoL-1 cells to fMLP, C5a, and PAF was shown to be regulated independently. EoL-1 cells and dbcAMP seem to be useful for examining chemotactic receptor expression and its signal transduction mechanisms.