Lipopolysaccharide-induced stem cell factor messenger RNA expression and production in odontoblast-like cells.
Lipopolysaccharide-induced stem cell factor messenger RNA expression and production in odontoblast-like cells.
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DOI：
10.1016/j.joen.2011.10.030
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发表时间：
2012-05
影响因子：
4.2
通讯作者：
S. Oliveira;V. A. C. Santos
中科院分区：
文献类型：
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作者：
S. Oliveira;V. A. C. Santos
目的探讨脂多糖（lipopolysaccharide，LPS）刺激成牙本质细胞（odontoblast，OD）样细胞（MDPC-23）表达和分泌干细胞因子（stem cell factor，SCF）的机制，并探讨此过程中激活的信号转导途径。SCF在OD样细胞的表达进行了分析，通过逆转录聚合酶链反应，SCF生产进行了评估，通过酶联免疫吸附试验。在另一组实验中，OD样细胞用地塞米松（DEX）、MK 886（MK）、p42/44抑制剂（PD 98059 [PD]），p38抑制剂（SB 202190 [SB]），或pi 3 k抑制剂（wortmannin [Wort]）处理30分钟，然后用LPS刺激LPS（0.1 μ g/mL）刺激1h可诱导SCF表达，但随着LPS浓度的增加（1、10和100 μg/mL），SCF表达量下降。DEX和MK能抑制SCF mRNA的表达。结论SLPS诱导OD样细胞中SCF mRNA的表达和产生是通过白三烯的产生或细胞因子和/或趋化因子的形成，激活p42/44、p38和PI 3 K通路。数据表明，SCF释放的OD样细胞可能作为免疫应答调节剂。
INTRODUCTIONThis study was done to evaluate the mechanism involved in stem cell factor (SCF) expression and production by lipopolysaccharide (LPS)-stimulated odontoblast (OD)-like cells and to investigate the signal transduction pathway activated in the process.METHODSODs-like cells (MDPC-23) were stimulated with different LPS concentrations for 1, 6, and 24 hours. SCF expression in OD-like cells was analyzed by reverse-transcriptase polymerase chain reaction, and SCF production was assessed by enzyme-linked immunosorbent assay. In another set of experiments, OD-like cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD 98059 [PD]), p38 inhibitor (SB 202190 [SB]), or PI3K inhibitor (wortmannin [Wort]) for 30 minutes followed by stimulation with LPS (0.1 μg/mL) for 1 hour.RESULTSOD-like cells stimulated with LPS (0.1 μg/mL) for 1 hour expressed SCF, but SCF production decreased with increasing LPS concentrations (1, 10, and 100 μg/mL). DEX and MK were able to inhibit SCF messenger RNA (mRNA) expression. PD, SB, and Wort inhibited the SCF mRNA expression.CONCLUSIONSLPS-induced SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways. Data suggest that SCF released by OD-like cells may act as immune response modulators.