The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
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DOI：
10.1016/s0092-8674(03)00003-5
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发表时间：
2003-01-24
期刊：
影响因子：
64.5
通讯作者：
Jürgens, G
中科院分区：
文献类型：
--
作者：
Geldner, N;Anders, N;Jürgens, G
ARF GTP酶的交换因子（ARF-GEFs）调节多种生物体中的囊泡运输。拟南芥蛋白质GNOME是一种对布雷菲德菌素A（Brefeldin A，BFA）敏感的ARF-GEF，是植物激素生长素的候选转运蛋白PIN 1正确极性定位所必需的。GnOM突变导致发育缺陷，类似于干扰生长素运输所引起的发育缺陷。PIN 1定位和生长素转运也对BFA敏感。在本文中，我们表明，GNOME本地化的内涵体，并要求其结构的完整性。我们设计了一个抗BFA的GNOME版本。在含有这种全功能的GNOME变体的植物中，PIN 1定位和生长素运输对BFA不再敏感，而其他蛋白质的运输仍然受到药物的影响。我们的研究结果表明，生长素转运组分的回收需要GNOME，并建议ARF-GEFs调节特定的内体运输途径。
Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to developmental defects that resemble those caused by interfering with auxin transport. Both PIN1 localization and auxin transport are also sensitive to BFA. In this paper, we show that GNOM localizes to endosomes and is required for their structural integrity. We engineered a BFA-resistant version of GNOM. In plants harboring this fully functional GNOM variant, PIN1 localization and auxin transport are no longer sensitive to BFA, while trafficking of other proteins is still affected by the drug. Our results demonstrate that GNOM is required for the recycling of auxin transport components and suggest that ARF-GEFs regulate specific endosomal trafficking pathways.