Fast direct melting of brackish sea-ice samples results in biologically more accurate results than slow buffered melting
Fast direct melting of brackish sea-ice samples results in biologically more accurate results than slow buffered melting
复制标题
咸海冰样本的快速直接融化比缓慢缓冲融化产生的生物学结果更准确
DOI：
10.1007/s00300-014-1563-1
复制
发表时间：
2014
期刊：
影响因子：
1.7
通讯作者：
R. Autio
中科院分区：
文献类型：
--
作者：
Janne;J. Piiparinen;J. Blomster;M. Majaneva;S. Müller;J. Uusikivi;R. Autio
用于生物分析的海冰样品，如叶绿素-a、藻类和原生动物的细胞计数和初级生产，受到采样和样品处理方法的影响。在本研究中，我们通过在两种温度[+4°C和室温[RT]下以不同方式（直接融化、过滤海水缓冲融化（FSW）和添加营养物的两种不同盐度的人工海水缓冲融化）融化波罗的海冰样品，比较了不同的样品处理方法。我们表明，用于最常用的生物分析的海冰样品可以在不添加FSW的情况下融化。尤其要避免添加人工海水。为了最大限度地减少生物过程，如生长、死亡、捕食和色素降解，熔化应在RT下快速完成，最好轻轻摇动样品以保持熔体冷却。
Sea-ice samples intended for biological analyses, e.g., chlorophyll-a, cell enumeration of algae and protozoa and primary production, are affected by the sampling and sample processing methods. In this study, we compared different sample processing methods by melting Baltic Sea ice samples in different ways (direct melting, buffered melting in filtered seawater (FSW) and buffered melting in artificial seawater at two different salinities with added nutrients) at two temperatures [+4 °C and room temperature (RT)]. We show that sea-ice samples intended for most commonly used biological analyses can be melted without the addition of FSW. In particular, adding artificial seawater should be avoided. To minimize biological processes, such as growth, death, predation and pigment degradation, the melting should be done rapidly at RT preferably by gently shaking the sample to keep the melt cool.