Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)
Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1677/joe.1.06142
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发表时间：
2005-07-01
影响因子：
4
通讯作者：
Moon, TW
中科院分区：
文献类型：
--
作者：
Doyon, C;Trudeau, VL;Moon, TW
本研究的目的是鉴定虹鳟鱼促肾上腺皮质激素释放因子结合蛋白(CRF-BP)的基因序列，并检测CRF-BP和CRF mRNA水平在不同胁迫强度下的变化。鲑鱼被一次或三次连续的追逐，直到筋疲力尽，然后恢复2小时，这是身体上的干扰。重复追逐运动后，大鼠脑垂体CRF-BP和视前区CkF1mRNA含量才显著增加。身体障碍增加了血浆皮质醇水平，其中变化最大的是暴露在重复追逐事件中的那组鲑鱼。将鲑鱼单独隔离在INI罐中或限制在1-51箱中4、24或72h，72h时封闭鱼的CRF-BP基因表达水平高于隔离鱼，但与对照组相比差异无统计学意义。与隔离的鲑鱼相比，封闭的鲑鱼视前区CRF1基因的表达水平较高，并持续较长时间的升高。隔离导致血浆皮质醇水平短暂升高，但在被限制的鱼中产生更高的皮质醇水平，这表明这种处理比隔离更有压力。这些结果表明，应激的强度和持续时间是调节虹鳟鱼CRF和CRF-BP rnRNA水平的重要因素。我们推测，垂体CRF-BP参与调节应激轴的活动，可能是通过减少对皮质激素受体CRF1的获取。
The objectives of this study were to characterize rainbow trout (Oncorhynchus mykiss) corticotropin-releasing factor (CRF)-binding protein (CRF-BP) cDNA and to examine the variations in CRF-BP and CRF mRNA levels in response to different intensities of stress. Trout were physically disturbed by a single or three consecutive periods of chasing until exhaustion followed by 2 h of recovery. The pituitary CRF-BP and preoptic area CkF1 mRNA contents were significantly increased only after repeated chasing events. Physical disturbance increased plasma cortisol levels with the largest change occurring in the group of trout that were exposed to repeated chasing events. Trout were also individually isolated in IN I tanks or confined to 1-5 1 boxes for 4, 24 or 72 h. CRF-BP mRNA levels in confined fish were greater than those of isolated fish at 72 h although there were no differences compared with the control group. CRF1 mRNA levels in the preoptic area were greater and remained elevated for a longer period in confined compared with isolated trout. Isolation led to a transient increase in plasma cortisol levels, but the higher cortisol values developed in the confined fish suggest that this treatment was more stressful than isolation. These results demonstrate that the intensity and duration of stress are important factors regulating CRF and CRF-BP rnRNA levels in rainbow trout. We hypothesize that pituitary CRF-BP is involved in regulating the activity of the stress axis, possibly by reducing access to CRF1 receptors in the corticotropes.