Diabetes-impaired wound healing and altered macrophage activation: A possible pathophysiologic correlation
Diabetes-impaired wound healing and altered macrophage activation: A possible pathophysiologic correlation
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糖尿病损伤的伤口愈合和改变的巨噬细胞活化：可能的病理生理学相关性
DOI：
10.1111/j.1524-475x.2012.00772.x
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发表时间：
2012-03-01
影响因子：
2.9
通讯作者：
Lu, Shuliang
中科院分区：
文献类型：
--
作者：
Miao, Mingyuan;Niu, Yiwen;Lu, Shuliang
巨噬细胞在伤口愈合中起着至关重要的作用，在体外可以被激活为两种不同的表型：经典巨噬细胞激活（caM）和替代巨噬细胞激活（aaM）。本研究探讨了链脲佐菌素诱导的糖尿病大鼠皮肤修复受损是否与巨噬细胞激活改变有关。我们的研究结果表明，通过双免疫染色可以观察到巨噬细胞在伤口愈合过程中的活化表型。caM巨噬细胞出现在创面愈合初期，其次是aaM巨噬细胞，在正常创面中以aaM巨噬细胞为主。然而，通过免疫印迹和实时聚合酶链反应（PCR）检测与激活相关的标志物，糖尿病伤口在早期表现为caM不足，而在增殖后期表现为aaM过量。此外，两组巨噬细胞激活标志物均与指导性T辅助细胞1型(Th1)/Th2细胞因子相关。研究表明，巨噬细胞激活的改变可能导致糖尿病伤口愈合受损，而恢复这种异常激活的策略可能有助于促进伤口愈合过程。
Macrophages play a critical role in wound healing and can be activated to two distinctive phenotypes in vitro: classical macrophage activation (caM) and alternative macrophage activation (aaM). This study investigated whether the impaired cutaneous repair observed in streptozotocin-induced diabetic rats was associated with altered macrophage activation. Our results show that macrophage activation phenotypes could be observed in wound healing through double immunostaining. The caM macrophages appeared in the initial stage of wound healing, followed by aaM macrophages, which predominated in normal wounds. However, through examining markers associated with activation by immunoblotting and real-time polymerase chain reaction (PCR), diabetic wounds demonstrated insufficient caM in the early stage but excessive aaM in the later proliferative phase. Moreover, the macrophage activation markers were correlated with the instructive T helper cell type 1 (Th1)/Th2 cytokines in both groups. It was indicated that changed macrophage activation might contribute to impaired healing in diabetes wounds, and that strategies for reverting this abnormal activation could be useful for enhancing the wound healing process.