Molecular characterization of a novel bovine group A rotavirus
Molecular characterization of a novel bovine group A rotavirus
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DOI：
10.1016/j.vetmic.2007.02.010
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发表时间：
2007-07-20
影响因子：
3.3
通讯作者：
Inoue, Kyoichi
中科院分区：
文献类型：
--
作者：
Fukai, Katsuhiko;Takahashi, Takashi;Inoue, Kyoichi
本研究通过对编码VP5*和VP7的基因组片段的部分序列分析，确定了一种新的牛A组轮状病毒，即Tak2，该病毒是从日本赤木县成年牛腹泻中分离到的。编码VP5*的基因组片段和VP7的一半氨基端部分的核苷酸(NT)和推导的氨基酸(AA)序列与先前发表的携带P和G型特异性的A组轮状病毒的序列同源性较低(VP5*：NT同源性)。61.6%-67.6%和氨基酸同源性58.0%-71.4%；VP7氨基末端一半：核苷酸同源性57.8%-73.5%，氨基酸同源性61.2%-70.9%)。此外，对编码VP5*的基因组片段和VP7的一半氨基末端部分的核苷酸序列的系统发育分析表明，Tak2形成了一个独立于已建立的P和G型的分支。这些结果表明，Tak2可能具有A组轮状病毒中尚未报道的新的P和G型。(C)2007 Elsevier B.V.保留所有权利。
In this study, by partial sequence analysis of the genome segments encoding VP5* and VP7, we characterized a novel bovine group A rotavirus, namely, Tak2, that was detected from adult cattle diarrhea in Tochigi Prefecture, Japan. The nucleotide (nt) and deduced amino acid (aa) sequences of the genome segments encoding VP5* and half of the amino terminal portion of VP7 of Tak2 revealed a low identity with those of group A rotaviruses carrying previously published P and G type specificities (VP5*:nt identity. 61.6%-67.6% and aa identity, 58.0%-71.4%; half of the amino terminal portion of VP7: nt identity, 57.8%-73.5% and aa identity, 61.2%--70.9%). Additionally, phylogenetic analysis of the nt sequences of the genome segments encoding VP5* and half of the amino terminal portion of VP7 revealed that Tak2 formed a branch separate from the established P and G types. These results suggested that Tak2 could possess novel P and G types yet not reported among group A rotaviruses. (C) 2007 Elsevier B.V. All rights reserved.