VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles.
VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles.
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DOI：
10.1083/jcb.118.5.1003
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发表时间：
1992-09
期刊：
影响因子：
--
通讯作者：
Simons K
中科院分区：
文献类型：
--
作者：
Kurzchalia TV;Dupree P;Parton RG;Kellner R;Virta H;Lehnert M;Simons K
在简单的上皮细胞中，顶侧和基底侧蛋白质在递送到适当的质膜结构域之前被分选到单独的囊泡载体中。为了剖析假定的分选机制，我们用洗涤剂CHAPS溶解了高尔基体衍生的运输囊泡，并表明顶端标记物流感血凝素（HA）与几个完整的膜蛋白一起形成了一个大的复合物。值得注意的是，一组类似的CHAPS不溶性蛋白质被发现后溶解的总细胞膜部分。这允许克隆编码该复合物的一种蛋白质VIP 21（21kD的囊泡整合膜蛋白）的cDNA。瞬时表达的蛋白质出现在高尔基体、质膜和泡状结构上。我们建议，VIP 21是一个组成部分的分子机械囊泡运输。
In simple epithelial cells, apical and basolateral proteins are sorted into separate vesicular carriers before delivery to the appropriate plasma membrane domains. To dissect the putative sorting machinery, we have solubilized Golgi-derived transport vesicles with the detergent CHAPS and shown that an apical marker, influenza haemagglutinin (HA), formed a large complex together with several integral membrane proteins. Remarkably, a similar set of CHAPS-insoluble proteins was found after solubilization of a total cellular membrane fraction. This allowed the cloning of a cDNA encoding one protein of this complex, VIP21 (Vesicular Integral-membrane Protein of 21 kD). The transiently expressed protein appeared on the Golgi-apparatus, the plasma membrane and vesicular structures. We propose that VIP21 is a component of the molecular machinery of vesicular transport.