Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro.
Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro.
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DOI：
10.1152/ajpgi.00145.2006
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
S. Amemori;A. Ootani;S. Aoki;T. Fujise;R. Shimoda;T. Kakimoto;Ryosuke Shiraishi;Y. Sakata;
中科院分区：
文献类型：
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作者：
S. Amemori;A. Ootani;S. Aoki;T. Fujise;R. Shimoda;T. Kakimoto;Ryosuke Shiraishi;Y. Sakata;
肥胖是结肠癌的一个危险因素，它与血清瘦素水平的升高有关，瘦素是一种由脂肪细胞产生的蛋白质。本研究的目的是通过培养结肠癌细胞系来阐明脂肪组织对结肠癌增殖的影响。为此，在三维胶原凝胶培养系统中，将结肠癌细胞(CACO-2、T84和HT29)与脂肪组织、分离的成熟脂肪细胞和分离的前脂肪细胞共同培养。所用脂肪细胞和前脂肪细胞来自C57BL/6J和瘦素缺陷ob/ob小鼠。用核摄取溴脱氧尿嘧啶核苷来评价癌细胞的增殖情况。从C57BL/6J小鼠分离的脂肪组织、成熟脂肪细胞和前脂肪细胞对结肠癌细胞的增殖有明显的促进作用。成熟脂肪细胞对癌细胞系的这种营养作用只在瘦子鼠的细胞上观察到，而对ob/ob小鼠的细胞没有观察到。相反，在ob/ob小鼠中，前脂肪细胞的营养作用并未被取消，这一发现得到了瘦素对癌细胞具有营养作用的结果的支持。总之，脂肪细胞在体外能够促进结肠癌细胞的增殖，部分是通过瘦素，提示脂肪组织，包括成熟脂肪细胞和前脂肪细胞，可能促进结直肠癌的生长。
Obesity, a risk factor for colon cancer, is associated with elevated serum levels of leptin, a protein produced by adipocytes. The aim of the present study was to clarify the effects of adipose tissue on colon cancer proliferation by using cultured cell lines. To achieve this, colon cancer cells (CACO-2, T84, and HT29) were cocultured with adipose tissue, isolated mature adipocytes, and isolated preadipocytes in a three-dimensional collagen gel culture system. The adipocytes and preadipocytes used were isolated from C57BL/6J and leptin-deficient ob/ob mice. Proliferation of the cancer cells was evaluated by nuclear bromodeoxyuridine uptake. The adipose tissue, mature adipocytes, and preadipocytes isolated from C57BL/6J mice significantly increased the proliferation of the colon cancer cells. This trophic effect of mature adipocytes on the cancer cell lines was observed only for cells from lean littermates and not for those from ob/ob mice. In contrast, the trophic effect of preadipocytes was not abolished in ob/ob mice, and this finding was supported by the result that leptin had a trophic effect on cancer cells. In conclusion, adipocytes were able to enhance the proliferation of colon cancer cells in vitro, partly via leptin, suggesting that adipose tissues, including mature adipocytes and preadipocytes, may promote the growth of colorectal cancer.