Genetic manipulation in chickens
Genetic manipulation in chickens
复制标题
鸡的基因操作
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
M. Naito
中科院分区：
文献类型：
--
作者：
M. Naito
鸡胚培养技术的发展为早期鸡胚的操作提供了精确的方法，并使分析孵化鸡的操作结果成为可能。人们已经尝试将外源DNA引入鸡的种系，但通过非病毒方法生产转基因鸡的有效方法尚未被设计出来。由于生产种系嵌合鸡的系统是通过从早期胚胎中分离的原始生殖细胞的转移而建立的，因此对原始生殖细胞的操作似乎是最有前途的鸡遗传操作方法之一。
Development of chicken embryo culture techniques provides precise methods for the manipulation of early chicken embryos and has made it possible to analyse the results of the manipulation in hatched chickens. Various attempts have been made to introduce exogenous DNA into the chicken germline, but an efficient method for producing transgenic chickens by non-viral methods has not yet been devised. Since the system for producing germline chimaeric chickens has been established by the transfer of primordial germ cells isolated from early stage embryos, manipulation of primordial germ cells seems to be one of the most promising methods for genetic manipulation in chickens.