Biodiversity of the mucosa-associated microbiota is stable along the distal digestive tract in healthy individuals and patients with IBD
Biodiversity of the mucosa-associated microbiota is stable along the distal digestive tract in healthy individuals and patients with IBD
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DOI：
10.1097/01.mib.0000159662.62651.06
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发表时间：
2005-05-01
影响因子：
4.9
通讯作者：
Doré, J
中科院分区：
文献类型：
--
作者：
Lepage, P;Seksik, P;Doré, J
背景资料：与炎症性肠病（IBD）相关的炎症过程非常接近的粘膜相关微生物群可能具有致病作用。我们使用一种非培养的方法来分析IBD患者远端消化道不同部位的粘膜相关微生物群。方法：35例患者（20例克罗恩病，11例溃疡性结肠炎）。和4名对照）进行结肠镜检查。活检（n = 126）取自4个部位：回肠、右结肠、左结肠和直肠。还从7个个体获得粪便样品。利用16 SrDNA的时间温度梯度凝胶电泳（TTGE）分析了优势种的多样性。TTGE配置文件进行了比较，使用软件来衡量的相似度。结果：在一个Liven的个人，4段远端消化道之间的整体相似性百分比为94.7 +/-4.0%，无论临床状态。在总体群体中，给定片段的所有特征的平均相似性为59.3 +/- 18.3%。聚类分析显示TTGE图谱与临床状态无聚类关系。4个采样点的优势菌群与肠道黏膜相关菌群的相似性均小于92%。结论：肠道黏膜相关菌群与肠道黏膜相关菌群的优势菌群不同。他们还表明，在给定的个体中，微生物群沿远端消化道沿着相对稳定，在健康受试者和IBD患者中显示从回肠到直肠的优势物种多样性的轻微演变。
Background: The mucosa-associated microbiota, being very close to the inflammatory process associated with inflammatory bowel disease (IBD), may have a pathogenic role. We used a culture-independent method to analyze the mucosa-associated microbiota in IBD patients at various points of the distal digestive tract.Methods: Thirty-five patients (20 with Crohn's disease, 11 with ulcerative colitis. and 4 controls) underwent colonoscopy. Biopsies (n = 126) were taken from 4 sites: the ileum, right colon, left colon, and rectum. Fecal samples were also obtained from 7 individuals. Temporal temperature gradient gel electrophoresis (TTGE) of 16S rDNA was used to evaluate dominant species diversity. TTGE profiles were compared using software that measures the degree of similarity.Results: In a Liven individual, the overall similarity percentage between the 4 segments of the distal digestive tract was 94.7 +/- 4.0%, regardless of clinical status. The average similarity of all profiles for a given segment was 59.3 +/- 18.3% in the overall Population. Dendrogram analysis showed that TTGE profiles did not cluster with clinical Status. Differences were observed between the dominant fecal microbiota and the mucosa-associated microbiota of all 4 sites, with similarity percentages less than 92%.Conclusions: These results confirm that the dominant species differ between the mucosa-associated and fecal microbiota. They also show that, in a given individual, the inicrobiota is relatively stable along the distal digestive tract, showing a slight evolution in dominant species diversity from the ileum to the rectum, in both healthy subjects and patients with IBD.