Uremic anorexia and ghrelin expression in the amygdala
Uremic anorexia and ghrelin expression in the amygdala
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DOI：
10.1016/j.neulet.2012.08.040
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发表时间：
2012-10
影响因子：
2.5
通讯作者：
Rongguo Fu;Rong-liang Xue;Jing Wang;Liqun Ma;Jianghua Lv;Li Wang;Ganglian Yao;Heng Ge;Zhao Chen;Z. Duan;Yarong Wang
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文献类型：
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作者：
Rongguo Fu;Rong-liang Xue;Jing Wang;Liqun Ma;Jianghua Lv;Li Wang;Ganglian Yao;Heng Ge;Zhao Chen;Z. Duan;Yarong Wang
背景/目的：促生长素释放肽可以作为进餐时饥饿和进餐开始的信号。杏仁核在习得性情绪激发的食欲行为中不可或缺。为探讨慢性肾功能衰竭（CRF）大鼠杏仁核ghrelin的变化及其与尿毒症性厌食的关系，将大鼠随机分为CRF组和对照组，每组16只。采用5/6肾切除术建立CRF模型。当CRF组血浆肌酐（PCr）和血尿素氮（BUN）高于正常值2倍时，测定大鼠摄食量（g/24 h），然后处死大鼠，采用免疫组化法检测杏仁核ghrelin蛋白表达，RT-PCT法检测ghrelin mRNA表达。术后8周时，CRF大鼠BUN、PCr均超过正常值的1倍，进食量明显低于对照组（P<0.05）。CRF组大鼠杏仁核ghrelin蛋白和mRNA表达均明显减少，且与摄食量减少呈正相关（P<0.05）。结论CRF大鼠杏仁核ghrelin蛋白和mRNA表达减少可能与尿毒症性厌食有关。
BACKGROUND/AIMSGhrelin can act as a signal for mealtime hunger and meal initiation. Amygdala is indispensable in appetitive behavior motivated by learned emotions. This study was to investigate the alteration of ghrelin in the amygdala of rats with chronic renal failure (CRF) and its relation with uremic anorexia.METHODSSD rats were randomly classified into CRF group and control group (n=16 per group). The CRF model was constructed using 5/6 nephrectomy. When plasma creatinine (PCr) and blood urea nitrogen (BUN) in the CRF group were twice more than the normal level, food intake (g/24h) was measured and then all rats were killed for detection of ghrelin protein expression in the amygdala using immunohistochemical analysis and mRNA expression using RT-PCT. Statistics was conducted with one-way analysis of variance, Student–Newman–Keuls-q test and correlation analysis.RESULTSBy the 8th week after the surgery, the BUN and PCr of CRF rats exceeded double the normal level, and their food intake was obviously decreased compared with the controls (P<0.05). The protein and mRNA expression of ghrelin in the amygdala of CRF group were significantly reduced, and there was a positive correlation between this reduction and the decrease in food intake (P<0.05).CONCLUSIONThe reduction of amygdala's ghrelin in CRF rats may be associated with uremic anorexia.