G0, A GUANINE NUCLEOTIDE-BINDING PROTEIN - IMMUNOHISTOCHEMICAL LOCALIZATION IN RAT-BRAIN RESEMBLES DISTRIBUTION OF 2ND MESSENGER SYSTEMS
G0, A GUANINE NUCLEOTIDE-BINDING PROTEIN - IMMUNOHISTOCHEMICAL LOCALIZATION IN RAT-BRAIN RESEMBLES DISTRIBUTION OF 2ND MESSENGER SYSTEMS
复制标题
DOI：
10.1073/pnas.83.12.4561
复制
发表时间：
1986-06-01
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
WORLEY, PF;BARABAN, JM;SNYDER, SH
我们使用针对Go的α39亚基的选择性多克隆抗血清，通过免疫组织化学在大鼠脑中定位了鸟嘌呤核苷酸结合蛋白Go。特异性染色分布广泛，在神经毡中丰富，在神经元细胞体中不存在，并且表现出区域异质性。大脑皮层的染色丰富，特别是分子层、海马结构的神经纤维、纹状体、黑质网状部、小脑的分子层、脊髓的胶质质和垂体后叶。黑质中的高密度染色反映了包含 Go 的纹状体黑质通路，因为纹状体病变减少了网状部的同侧免疫染色。确认黑质膜与百日咳毒素的免疫染色、定量[32P]ADP-核糖基化表明66%.+-。纹状体病变同侧 Go 减少 11%（平均 .+-.SEM）。 Go可能与小脑中的浦肯野细胞有关，因为出生后失去浦肯野细胞的突变小鼠（神经型）的细胞膜中的百日咳毒素底物明显减少。 Go 的定位在许多区域与蛋白激酶 C（磷脂酰肌醇循环的一个组成部分）的定位相对应，表明 Go 在大脑中与磷脂酰肌醇循环的调节相关的主要作用。
We have localized a guanine nucleotide-binding protein, Go, in rat brain by immunohistochemistry with a selective polyclonal antiserum to the .alpha.39 subunit of Go. Specific staining is widely distributed, abundant in neuropil, absent from neuronal cell bodies, and displays regional heterogeneity. Staining is enriched in cerebral cortex, particularly the molecular layer, neuropil of the hippocampal formation, striatum, substantia nigra pars reticulata, molecular layer of the cerebellum, substantia gelatinosa of the spinal cord, and posterior pituitary. High density staining in the substantia nigra reflects a Go-containing striatonigral pathway since striatal lesions reduce ipsilateral immunostaining in pars reticulata. Confirming immunostaining, quantitative [32P]ADP-ribosylation of nigral membranes with pertussis toxin indicates a 66% .+-. 11% (mean .+-. SEM) reduction of Go ipsilateral to striatal lesions. Go may be associated with Purkinje cells in the cerebellum since membranes from mutant mice (Nervous), which postnatally lose Purkinje cells, are markedly depleted in pertussis toxin substrate. The localizations of Go correspond in many areas with those of protein kinase C, a component of the phosphatidylinositol cycle, suggesting a major role for Go in the brain related to regulation of the phosphatidylinositol cycle.