Diversity and methane oxidation of active epibiotic methanotrophs on live Shinkaia crosnieri
Diversity and methane oxidation of active epibiotic methanotrophs on live Shinkaia crosnieri
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DOI：
10.1038/ismej.2013.226
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Tomo-o Watsuji;Asami Yamamoto;Y. Takaki;K. Ueda;S. Kawagucci;K. Takai
中科院分区：
文献类型：
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作者：
Tomo-o Watsuji;Asami Yamamoto;Y. Takaki;K. Ueda;S. Kawagucci;K. Takai
Shinkaia crosnieri是一种galatheid蟹，主要生活在日本冲绳海槽的深海热液系统中。本研究对中国南方红豆杉刚毛表生微生物群落中活性甲烷氧化菌的系统发育多样性进行了研究。通过逆转录-聚合酶链反应（RT-PCR）对编码颗粒甲烷单加氧酶亚基的功能基因（pmoA）进行了表征。系统发育分析表明，S. crosnierisetae由Ia型和Ib型甲烷氧化菌组成。用定量RT-PCR方法测定了不同RNA稳定化方法对表生菌群落中pmoA和16 SrRNA转录本丰度的影响。我们的新的RNA固定方法在取样后立即进行，有效地保存了细胞RNA组合，特别是不稳定的mRNA群体，包括pmoAmRNA。生活中的甲烷消耗量。crosnieri也通过在大气和原位静水压力下的连续流培养来估计，并且提供了表生微生物群落的甲烷氧化活性的明确证据，其不受静水压力的显著影响。我们的研究揭示了重要的生态功能和营养贡献的表生甲烷氧化菌的优势。冲绳海槽深海热液系统中的crosnieripomulations。总之，我们的研究给出了明确的事实多样性和甲烷氧化的活性甲烷氧化菌在与无脊椎动物相关的表生群落。
Shinkaia crosnieriis a galatheid crab that predominantly dwells in deep-sea hydrothermal systems in the Okinawa Trough, Japan. In this study, the phylogenetic diversity of active methanotrophs in the epibiotic microbial community on the setae ofS. crosnieriwas characterized by reverse transcription-polymerase chain reaction (RT-PCR) of a functional gene (pmoA) encoding a subunit of particulate methane monooxygenase. Phylogenetic analysis ofpmoAtranscript sequences revealed that the active epibiotic methanotrophs onS. crosnierisetae consisted of gammaproteobacterial type Ia and Ib methanotrophs. The effect of different RNA stabilization procedures on the abundance ofpmoAand 16S rRNA transcripts in the epibiotic community was estimated by quantitative RT-PCR. Our novel RNA fixation method performed immediately after sampling effectively preserved cellular RNA assemblages, particularly labile mRNA populations, includingpmoAmRNA. Methane consumption in liveS. crosnieriwas also estimated by continuous-flow incubation under atmospheric andin situhydrostatic pressures, and provided a clear evidence of methane oxidation activity of the epibiotic microbial community, which was not significantly affected by hydrostatic pressure. Our study revealed the significant ecological function and nutritional contribution of epibiotic methanotrophs to the predominantS. crosnieripopulations in the Okinawa Trough deep-sea hydrothermal systems. In conclusion, our study gave clear facts about diversity and methane oxidation of active methanotrophs in the epibiotic community associated with invertebrates.